"Chromatography of chloroplast pigments" Essays and Research Papers

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    Explain in detail why palisade cells in plants contain many chloroplasts Palisade cells are cells which are found within the leaves of many plants. They contain chloroplasts‚ which convert the energy in light to chemical energy through photosynthesis. The cylindrical shape of palisade cells allows a large amount of light to be absorbed by the chloroplasts. Beneath the palisade mesophyll are the spongy mesophyll cells‚ irregularly-shaped cells that having many intercellular spaces to allow the

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    Organic Chem II Lab 2/15/15 Friedel-Crafts Acylation of Ferrocene and Column Chromatography Introduction: The intention of this lab is to analyze the formation of acetylferrocene using column chromatography. The Friedel-Crafts acylation reaction created acetylferrocene and diacetylferrocene‚ using phosphoric acid as a catalyst for the reaction between acetic anhydride and ferrocene (once applying heat). During column chromatography‚ a solution is passed through a filtration system of silica‚ sand‚ and

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    tested for by a possible employer include Cocaine (crack)‚ Amphetamines (crystal)‚ Opiates (codeine‚ morphine‚ heroin)‚ PCP (phencyclidine)‚ and Marijuana. Gas chromatography/mass spectrometry is used to test hair and urine samples of possible drug abusers or job applicants‚ and it is the best method for the testing of drug use. Gas chromatography and mass spectrometry are two different methods for identifying chemical substances‚ and the two instruments have be coupled together to perform a highly complementary

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    Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe

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    Separating Water Soluble and Acetone Soluble Inks through Chromatography Abstract: Chromatography is a term used to define a set of laboratory techniques for the separation of mixtures. Through Chromatography we analyzed complex mixtures. In our recent lab we analyzed complex mixtures. The purpose of this lab was to find whether the water soluble or acetone soluble liquid traveled farther. In our recent lab we measured the distance traveled by the different colored water and acetone soluble

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    Introduction Flash-Column Chromatography is another useful technique used to separate and purify complex mixtures of compounds. In this experiment‚ TLC is used to determine the best solvent system for the column. Due to Flash-Column Chromatography1 being an inexpensive technique‚ many scientist use this process for drug and natural product purification. Method By using the information from Experiment 6B‚ the TLC solvent system used was 30% EtOAc: 70% Hex. Experiment 7 was broken into two parts‚

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    Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter

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    by Laboratory Reagents‚ Legacy Product Code: S/0380/48‚ CAS Number:8047-15-2) were powdered and analyzed as potassium bromide (KBr) pellets using FTIR (Model-JASCO FT/IR 4100 LE‚ made in Japan; Range: 4000-400 cm-1) Prabhu et al. (2013). Gas Chromatography–Mass Spectrometry (GC–MS) analysis: Acid hydrolysis of saponin was carried out according to the method described by Metwally et al. (2012) with some modification. Saponin was hydrolyzed with 2 N HCL (6 hr at 100 o C) under reflux‚ the residue

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    Inheritance System of anthocyanin pigments in Brassica rapa. Introduction The observations of Brassica rapa in class revealed a variation in color. Brassica rapa‚ also called fast plants‚ was studied because as their name suggests‚ they are able to complete a lifecycle in a short period of time. (www.fastplants.org) The color variations we observed among the plants were that some had green stalks while others were purple. The pigment which gives plants their color is called anthocyanin. (www.carnivorousplants

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    Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012   Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-

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