Alkaline Phosphatase was isolated using various techniques. BCIP spot test was performed after the anion exchange chromatography was done and the fractions were collected. The picture of BCIP spot test of the 20 fractions can be seen in figure 1. The intensity of each spot was different from each other. The more intense color the spot was‚ the more concentrate the AP was. The fractions were chosen to combine together based on the intensity‚ which made the stage 4 enzyme. Different volumes in each
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CHAPTER 4 RESULTS AND DISCUSSION 4.1 Identification of camphor and menthol using gas chromatography – mass spectrometry (GC – MS) In this study‚ the presence of menthol and camphor were determined from 5 different locally produced medicinal topical ointment samples. Sample of Vick® VapoRub® and Hurix’s Massage Balm were purchased from local pharmacies‚ Bunga Cengkih Balm and Serai Balm samples were purchased from the grocery shop. Buah Pala Balm sample was purchased from the night market
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Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined
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Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted with the
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CHROMATOGRAPHY LAB Name ______________________ Date ________________ Lab # ________ Partner _____________________ Purpose: To separate a mixture into its components. To learn the technique of chromatography. Materials: filter paper 2.0 ml of vinegar Felt marker pens ruler 400 ml beaker pencil 10 ml graduated cylinder paper clip Water - H2O Safety: Wear goggles and apron during the entire lab. Clean up all materials
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Composition of Datura alba Seed Oil by Gas Chromatography Mass Spectrometry Muhammad Nasimullah Qureshi‚* Muhammad Siddique‚ Inayat-ur-Rahman and Farina Kanwal Medicinal Botanic Centre‚ PCSIR Laboratories Complex Peshawar‚ Pakistan Received July 9‚ 2010; Accepted November 23‚ 2010; Published Online December 14‚ 2010 Methyl ester derivatives of fatty acids were analyzed for the determination of the constituents of Datura seed oil. Gas chromatography coupled to mass spectrometer was used for these
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Leo Pigao Susan Andres Edeliza Caparas Marites Sanchez Arma Cantre Aurora Lazaro Alma Castaño ABSTRACT Flavonoid pigments make plants colorful. Due to the varied colors‚ the researchers used extracts of Anthurium spathe‚ Onion skin and Rose petals as an alternative highlighter pen pigment to commercially available highlighter. The preparations of pigments were treated differently for quality sake. One was by boiling the samples in 100ml denatured alcohol for 1-2 minutes and the other
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extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon adding a solvent mixture of hexane and acetone‚ the chlorophyll and carotene pigments can be
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Introduction The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis‚ based on starch production‚ which wavelengths of light are involved in photosynthesis‚ and identify plant pigments found in a plant leaf by means of paper chromatography. Life on Earth is dependent entirely on the energy from the Sun‚ not only to keep the planet at a suitable temperature but also to provide the energy required to sustain life. The energy of the Sun‚ in the form of
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Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
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