and thin layer chromatography of plant pigments CHEMISTRY 201L EXPT 04 PAGE 01 - 12 Noel Angelo P. Kalacas*‚ Hanna Mae Laluces‚ Ina Bianca Lanuza Department of Chemistry‚ College of Science *Corresponding author; e-mail: knight_BeNcH66@yahoo.com Abstract Chromatography is a powerful technique for separating and/or identifying the components in a mixture. There are different types of chromatography and each has its own strengths and weaknesses. In this experiment‚ pigments of the chili
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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spectrum) by James Clerk Maxwell; this included: cosmic rays‚ visible light‚ x-rays‚ and radio waves. For plants to use sunlight‚ they have to absorb it. So pigments absorb light energy. Chlorophyll is a pigment in leaves that reflect green light waves. This is the most important pigment in photosynthesis and there are also accessory pigments used in plants. Also in this lab there is the internal and external anatomy of a typical leaf. Materials and methods: The first procedure dealt with the
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Question 1: You have carried out an investigation into the pigments that make up chlorophyll. Use the chromatogram you obtained when carrying out this investigation. Clearly explain the separation technique of paper chromatography in terms of partition. Calculate the Rf value of one of the constituent pigments and compare it to the book value. A paper chromatography was used to separate the pigments that make up chlorophyll to analyse‚ identify‚ and quantify the components‚ using paper (stationary
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This experiment focused on extracting and separating pigments of Chloroplast. For the procedure green leaves were grinded in a mortar with some chemicals and the fluid was filtrated to use for further analysis. Stripes of this solution were put on a filter paper and later‚ after dried placed into a beacon of solvent. After this the chloroplast pigments were separated by the solvent into groups of more or less soluble pigments. Aim How many pigment types are present in a green leaf? It is hoped to
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All green parts of a plant have chloroplasts. However‚ the leaves are the major site of photosynthesis for most plants. There are about half a million chloroplasts per square millimeter of leaf surface. The color of a leaf comes from chlorophyll‚ the green pigment in the chloroplasts. Chlorophyll plays an important role in the absorption of light energy during photosynthesis. Chloroplasts are found mainly in mesophyll cells forming the tissues in the interior of the leaf. O2 exits and CO2 enters
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in the form of organic compounds. There are two phases in photosynthesis‚ the light reaction and dark reaction. The following experiment tested only the light reaction. Photosynthesis takes place on the chloroplast‚ and the chloroplast contains chlorophyll pigments which absorb light energy. Pigments are particular to different wavelengths. The following experiment helped determined the fastest photosynthetic reaction rate from wavelength ranging from 450‚ 545‚ 650‚ and 750 nm‚ and intensities 3‚ 7
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Experiments Determination of osmotic potential Use of microscope‚ magnification‚ and measurement of cell size Observation of cells (a) animal cell: cheek cell (b) plant cell: leaf epidermis cell Enzyme activity Separation of photosynthetic pigments using paper chromatography Examining slides of transverse sections of C3 and C4 leaves Use of yeast in respiratory experiment Dissection of the mammalian digestive system Dissection of the mammalian respiratory system Examining slides of transverse sections of
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Chromatography Lab Questions Pre-Lab Questions 1. A pencil is used rather than a pen to mark the “starting line” in step two because a pen or marker while be used to place a small dot of ink in the center of the X that is made with a pencil. 2. Capillary action is filling of pores in a solid with a liquid. Capillary action is caused by spotting or absorption and is allowed to seep or flow through. 3. The type of pen that will probably give better separation in this paper chromatography
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green pigment chlorophyll. Chlorophyll molecules located in the thylakoid membrane of the chloroplasts are arranged into complexes known as photosystems. Each photosystem contains the reaction center complex and is surrounded by light harvesting complexes. The light harvesting complexes are composed of pigment molecules such as chlorophyll a‚ b and accessory pigments that broaden the spectrum of light absorbed for photosynthesis (Campbell‚ pg. 194). When a photo is absorbed by these pigments‚ the
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