EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
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Photosynthesis (happens in chloroplasts in mesophyll cells) 1. Light dependent stage Happens in the grana of chloroplasts Light (energy) gets trapped by chlorophyll pigments (light energy converted to chemical energy) This energy used to turn ADP and Pi into ATP Energy used to split water (H2O) into Hydrogen and Oxygen The hydrogen ions get collected by NADP‚ which becomes NADPH The Oxygen is waste‚ and leaves! So‚ in this stage‚ water comes in. ATP and NAPDH come out‚ as does Oxygen.
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Light Reactions in Isolated Chloroplasts Racquel Currie University of Minnesota Minneapolis‚ Minnesota Hypothesis If isolated chloroplasts are boiled‚ the enzymes that occur in photosynthesis are disrupted and the DCIP will not reduce. Chloroplasts are a type of plastid found within the plant they are the basis of photosynthesis. Without photosynthesis there would be no life on earth. Photosynthesis takes the energy from sunlight and coverts it into a chemical energy that can be used by
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When comparing and contrasting the mitochondria and chloroplast‚ a person learns all of the similarities and differences between the two. In this essay‚ you will read about the mitochondria‚ the chloroplast‚ and their biochemical reactions. The mitochondria often referred to as the powerhouse cell is found in the eukaruotic cells. There‚ those cells are often found in groups of hundreds. Mitochondria cells can be anywhere from 1 to 10 um long in length‚ but are able to change shapes‚ move‚ and divide
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was to separate plant pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment in chloroplasts. The chemical equation
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& Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation. • Use evidence
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Read on the find out why plants are truly the cornerstone of life. Model 1: An Overview of Photosynthesis and Chloroplast Structure 6CO2 + 6H2O C6H12O6 + 6O2 Site of the Calvin cycle‚ or light independent reactions 6 H 2O + 6 CO2 Contains chlorophyll. This is the site of the light dependent reactions 6 O2 Glucose (C6H12O6) moved to the leaf for transport 1. Which part of the chloroplast contains chlorophyll? 2. Where does the CO2 go? 3. Where is the energy from the sunlight used? 4. Name this process
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Plant Pigment Lab Question: What pigments are present in Red Wandering Jews? Background: A leaf is filled with many pigments. The pigments are usually masked by the prevalence of the green chlorophyll. Anothocyanin(red or purple)‚ carotene(orange)‚ and xanthrophyll(yellow) are found in different proportions in different leaves. Leaf pigments can be separated by using paper chromatography. Paper chromatography is a technique that extracts pigments into a paper filter called chromatogram. What are
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
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