Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of
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Chapter 1 THE PROBLEM AND ITS BACKGROUND Introduction The development of dyes to stain microorganisms was a significant advance in microbiology. Stains serve purposes such as it differentiate microorganisms from their surrounding environment and it allows detailed observation of microbial structures at high magnification. (http://inst.bact.wisc.edu/‚ © 2006-2013 Microbiology Laboratories) Gunasekaran (2005) defined staining as the method of artificially producing color in microorganisms to allow
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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Alkaline Phosphatase was isolated using various techniques. BCIP spot test was performed after the anion exchange chromatography was done and the fractions were collected. The picture of BCIP spot test of the 20 fractions can be seen in figure 1. The intensity of each spot was different from each other. The more intense color the spot was‚ the more concentrate the AP was. The fractions were chosen to combine together based on the intensity‚ which made the stage 4 enzyme. Different volumes in each
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Can I print T Shirts using dye sublimation printing? Dye sublimation printed shirts have started to become rather popular‚ sublimation tends to makes the t shirts more wearable and comparably soft. Dye sublimation printer is basically a computerized printer that turns the printed image‚ mirror reverse‚ in the form of gas and with the use of heat transfers the gaseous dye on the cloth giving the print ‘a no hand feel’. This process makes the most vivid and real images to be printed on the t shirts
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goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used in
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Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it
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sappan) WOOD AS TEXTILE DYE PRESENTED IN THE SCIENCE AND TECHNOLOGY DEPARTMENT (SCIENCE CURRICULUM) ILIGAN CITY NATIONAL HIGH SCHOOL ILIGAN CITY SUBMITTED TO: MRS. ANNALLEE Q. ARON RESEARCH TEACHER SUBMITTED BY: Ibrahim‚ Mahalia Taskeen R. Cabugatan‚ Fahad M. Lomansoc‚ Jake G. Miano‚ Ryan I. Surmion‚ Paul Jeffrey S. ABSTRACT Dyes known to the ancients came from plants such as indigo and madder or from the shells of mollusks; today most dyes are made from coal tar
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Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains Azucena‚ Alexis G. An Official Entry to 2010 Regional Science Fair Cluster 2 Physical Science- Individual Negros Occidental National Science High School January 13‚ 2011 Ma. Melanie P. Romero Research IIB Adviser APPROVAL SHEET The research paper attached hereto‚ entitled‚ “Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains”‚ prepared and submitted
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Lab #3 Separating Metal Cations by Paper Chromatography Introduction/Purpose: In chemistry‚ it is important to effectively analyze the components in a given mixture. In order to do so‚ chemists will analyze a mixture either qualitatively or quantitatively using different methods and equipment. Qualitative analyses are subjective‚ and only gather “non-numerical” data such as the color‚ appearance‚ and smell of the components. This data
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