Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted with the
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Abstract on Determining the Photosynthetic Activity in Plants Using Different Wavelengths of Light using Chromatography and a Spectrophotometer In this experiment‚ the separation of particular pigments‚ contained within a fresh spinach leaf‚ were examined. Paper chromatography is the process of separating certain molecules‚ or pigment molecules‚ based upon their polarity. Four different pigments were examined from the spinach leaf: chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and carotene. Based
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CHROMATOGRAPHY LAB Name ______________________ Date ________________ Lab # ________ Partner _____________________ Purpose: To separate a mixture into its components. To learn the technique of chromatography. Materials: filter paper 2.0 ml of vinegar Felt marker pens ruler 400 ml beaker pencil 10 ml graduated cylinder paper clip Water - H2O Safety: Wear goggles and apron during the entire lab. Clean up all materials
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Composition of Datura alba Seed Oil by Gas Chromatography Mass Spectrometry Muhammad Nasimullah Qureshi‚* Muhammad Siddique‚ Inayat-ur-Rahman and Farina Kanwal Medicinal Botanic Centre‚ PCSIR Laboratories Complex Peshawar‚ Pakistan Received July 9‚ 2010; Accepted November 23‚ 2010; Published Online December 14‚ 2010 Methyl ester derivatives of fatty acids were analyzed for the determination of the constituents of Datura seed oil. Gas chromatography coupled to mass spectrometer was used for these
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Enzymes are proteins which serve to reduce the activation energy required for biological reactions (Russell and others 2010). This allows biologically important chemical reactions to occur rapidly enough to allow cells to carry out their life processes (Russell and others 2010). Enzymes are made of one or more polypeptide strands‚ which individually or as an associated complex take on a three-dimensional shape. When properly associated‚ these shapes form the active site and other supporting structures
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ABSTRACT Rising energy prices and environmental problems led to the increase in alcohol demands in many aspects‚ like in making fuels. Many people are doing researches about making their own product so as to lessen their everyday expenses. Right now‚ Filipino scientists are in think of the search for alternative fuels‚ with the experts from the University of the Philippines in both Diliman and Los Banos campuses searching all over the archipelago for energy sources from the forests to the bottom
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Candy Chromatography By: Avani Reddy 7th Grade Introduction Ever wondered why candies are different colors? Ever wondered why candies are different colors? Many candies contain colored dyes. Bags of M&Ms or Skittles contain candies of various colors. The labels tell us the names of the dyes used in the candies. But which dyes are used in which candies? We can answer this by dissolving the dyes out of the candies and separating them using a method called chromatography. Research
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PROCEDURE Part I Setting Up Experiment 2D: Separation of a mixture by Paper Chromatography Objectives: 1. To assemble and operate a paper chromatography apparatus 2. To study and the significance of Rf values 3. To test various food colorings and to calculate their Rf values 4. To compare measured Rf values with standard Rf values 5. To separate mixtures of food coloring into their components 6. To identify the components of mixtures by means of their Rf values Materials / Apparatus: See “Health
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distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further than polar substances in this process. The separation
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the sample. Uses for Chromatography: *Pharmaceutical company - can determine how much chemical is in a new product or food. *Hospital- Can detect blood or alcohol levels in a patient’s blood. *Law enforcement- Can compare a sample found at a crime from the suspect. *Environment agency- Can determine the levels of pollutant in the water supply. *Manufacturing Plant- Can purify a chemical needed to make a product. Chromatography is very important
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