separate Attempt all questions in SECTION A and only TWO questions in SECTION B SECTION A (45 marks) Answer all question in this section in the spaces provided Q1 Forensic scientists use paper chromatography to compare the inks from five different bank notes with the ink used to make legal bank notes. The results are shown as a chromatogram in Fig. 4.1. (a) Draw the apparatus that could be used to produce this chromatogram.
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of metals in a Galvanic cell. Science Buddies.org 21. Yeasty beasties-The effect of reaction rate on yeast fermentation 22. Suck It Up: Capillary Action of Water in Plants- dye in water with white carnations 23. What Color Are the Leaves Really Turning? colors of fall by separating plant pigments with paper chromatography 24. Attack of the Killer Cabbage Clones. How do you clone a plant 25. Can Water Plants Be Used to Determine Water Quality? 26. Are There Bugs Under Your Feet?- numbering the
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of protein was measured using the commercialized Bradford reagent as described by Bradford (1976). A standard curve was plotted in a range 100-1000ug/mL in a Bradford-compatible buffer using bovine serum albumin as substrate (Appendix A). 1mL of the dye solution was added to 20uL of the protein sample‚ mixed and incubated for 10 minutes at room temperature before measuring the absorbance at 595nm using the spectrophotometer. 3.6 Verification for the Homogeneity and Molecular Weight of the DNA Polymerase
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Separations ........ 413 16.2.5 Multidimensional Planar Chromatography (Unidimensional Multiple Development and Two-Dimensional Development)..... 415 16.2.6 Quantitative Evaluation................................................................ 418 16.3 Modern TLC Techniques in the Separation of Flavonoids ....................... 418 16.3.1 Overpressured-Layer Chromatography ........................................ 418 16.3.2 Rotation Planar Chromatography................................................
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Biological chemistry 1B Experiment 1: Thin layer chromatography [TLC] Aim The aim of this experiment is to apply the technique of TLC to first separate out a mixture of coloured dyes and then to do the same for selected range of amino acids. Procedure 2 MMs of each different colour were collected into separate beakers. Approximately 5 drops of water is added to each of the beakers containing the MMs and the tablets were stirred until
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equal * protein aggregates since the electrostatic repulsive forces are at a minimum. 4. Precipitation with organic solvents or acidic protein precipitants -addition of O.S decreases dielectric constant PURiFICATION 1. Column chromatography 2. Density gradient centrifugation 3. Electrophoresis 4. enzymatic digestion 5. Removal of small ions or molecules or to exchange one ionic environment for another during purification is done by : Dialysis‚ Ultrafiltration and
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ABSTRACT At First my heartiest air towards to ALLAH‚ then thanks to our course teacher Mr.Rajib saha who give me chance to work regarding Detergent. A detergent is an agent used for cleaning. Detergents are synthetic surfactants‚ which is produced from petrochemicals rather than fatty acids and oils. They are very effective in hard‚ soft as well as salt water. Detergents are the salts of long chain of hydrocarbons such as alkyl sulphates. A detergent has several advantages over soaps in which we
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Recrystallization Experiment 2: Recrystallization & Melting Point • Most important method for the purification of organic solids • Separation of compounds based on differences in solubility between the compound of interest and its contaminants • Basic technique: 1. dissolve impure sample in an "appropriate" hot solvent Part A: Choosing a Solvent Part B: Purification of Phenacetin 2. cool solution slowly to induce crystal growth 3. filter resulting mixture to isolate crystals Reading:
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could be regarded as the chemistry of life. Organic Chemistry is of great medical‚ economic and technological importance. It touches almost every areas of our daily life (food‚ medicinal drugs‚ paper‚ ink‚ paint‚ plastic‚ fuel‚ textiles‚ pesticides‚ dyes‚ explosives‚ etc). Living organisms are nature’s laboratory where many chemical transformations are taking place independently‚ simultaneously and continuously. Hence most organic sample are mixtures of compounds. However‚ the need for
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What is a drug? A drug is a chemical substance that‚ when absorbed into the body‚ can alter normal bodily function. Many drugs have been banned in sport if they are deemed to provide an unfair advantage‚ pose a health risk‚ or are seen to violate the ‘spirit of sport’. The use of banned drugs by athletes is referred to as ‘doping’. The International Olympic Committee (IOC)‚ and more recently‚ the World Anti-Doping Agency (WADA) have been leading the way in the battle against drugs in sport.
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