Monosaccharide also called SIMPLE SUGAR‚ any of the basic compounds that serve as the building blocks of carbohydrates. Monosaccharides are polyhydroxy aldehydes or ketones; that is‚ they are molecules with more than one hydroxyl group (-OH)‚ and a carbonyl group (C=O) either at the terminal carbon atom (aldose) or at the second carbon atom (ketose). The carbonyl group combines in aqueous solution with one hydroxyl group to form a cyclic compound (hemi-acetal or hemi-ketal). Monosaccharides are
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Special Report Methyl ethyl ketone – A techno-commercial profile M ethyl ethyl ketone (MEK or 2-butanone) is an organic compound with the formula CH3C(O)CH2CH3. Its CAS number is 78-93-3. The colorless liquid ketone has a sharp‚ sweet odour of butterscotch and acetone. It is produced industrially on a large scale‚ and also occurs in trace amounts in nature. It is soluble in water and is commonly used as an industrial solvent. With natural and synthetic resins‚ MEK produces solutions with low viscosity
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at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion. This means that the affinity chromatography removed a big percentage of contaminating proteins. However‚ removing this huge amount of protein left us with a small amount of LDH
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Analysis of Ethanol in Moonshine | Using the GC-MS | Jennifer Greene | ------------------------------------------------- 4 May 2012 ------------------------------------------------- 4 May 2012 Introduction: In this experiment gas chromatography is applied to separate the water-ethanol mixture. This method is often used in the determination of alcohol in blood or urine. One obvious application is when law enforcement agencies need to determine whether or not someone is inebriated. In
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best. The idea is to be able to isolate the protien that is in interest from the protiens that are not needed. Below are several chromatography techniques that are used to analyze proteins. Size exclusion chromatography (SEC) In this type of technique the protein is separated according to the size of the protein. The matrixes used in size exclusion chromatography have a range of beads with different pore sizes (H. Dai). The seperation of the protein is dependent on how the protein can enter
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Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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Whiting was questioned about the disappearance of Payne‚ which had taken place about 8 km (5 miles) from Whiting’s place of residence. Whiting was routinely questioned as he had been placed on the Sex Offenders Register. The officers left Whiting‚ but were suspicious of his lack of concern for Payne‚ something that some of the worst offenders had shown when questioned in connection with Payne’s disappearance.[citation needed] When Whiting re-appeared soon after he attempted to drive away in his van
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