An electrophilic aromatic substitution reaction was performed on bromobenzene with nitric acid producing both 2-bromonitrobenzene and 4-bromonitrobenzene. Products of the reaction were purified through multiple recrystallizations and column chromatography creating multiple crops of a yellow powder. The percent yield of products was determined to be 51%. The melting point of Crop 1 was found to be 110-115 °C‚ and Crop 2 was found to be 37-90 °C indicating impurity in both crops. TLC analysis shows
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lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of two or more substances between a stationary
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An audit of these strategies is given in the writing (39). CE is an electrophoretic strategy that is often lumped with chromatographic routines in light of the fact that it shares a considerable lot of the basic necessities of chromatography. A wide scope of mixes can be determined utilizing TLC by utilizing diverse plates and versatile stages. GC is a helpful strategy for measurement. It can give the coveted determination‚ selectivity‚ and simplicity of evaluation. This strategy
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Relationship and biodiversity Introduction Botana curus is a valuable plant because it produces Curol‚ a compound used for treating certain kinds of cancer. Curol cannot be produced in the laboratory. Botana Curus grows very slowly and is on the endangered species list‚ so its ability to provide Curol in large quantities is limited. Species that are more closely related to Botana curus are more likely to produce the important substance Curol. Three similar plant species that are plentiful (X
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risk the possibility of death when their blood alcohol content ranges from 0.400% to 0.500%‚ or exceeds 0.500%. Qualitative and quantitative analysis of ethyl alcohol in postmortem specimens are commonly explored with the use of gas chromatography. Gas chromatography can assist in determining the amount of ethyl alcohol present at the time of death. This information could reveal whether a high level of ethyl alcohol contributed to the cause of death.
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EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine
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protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing and pooling. Stage 4 involves the Solvent/Detergent (S/D) viral inactivation which is the first dedicated viral clearance step. At Stage 5‚ the product undergoes cation exchange chromatography which aids in removing the S/D reagents
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Separation of Amino Acids by Paper Chromatography Chromatography is a common technique for separating chemical substances. The prefix “chroma‚” which suggests “color‚” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment‚ chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate and identify amino acids‚ the building blocks of proteins
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sure the apparatus did not fall apart while in use. For the next step of the experiment gas chromatography was used to analyze the samples. In gas chromatography‚ it puts the solution into two separate phases‚ the mobile (non-polar) and the stationary (polar) phase. The mobile phase is where the solution is carried by an inert gas‚ in this case helium‚ through a column that is heated. For gas chromatography‚ the 3 tubes of solution that we received were individually tested to determine its composition
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ORGANIC CHEMISTRY 1 LAB EXPERIMENT NO. 1 to 6 DISCUSSION EXPERIMENT NO. 1: Mel=ng Points And Boiling Points Of Organic Compounds Mel=ng Point -‐ temperature at which the liquid and solid are in equilibrium at a pressure of 1 atm Mel=ng Point Range -‐ determines the purity of a solid sample -‐ temp at
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