purification and physicochemical properties of tannase of Aspergillus oryzae. Sciences des Aliments‚ 10‚ 807–816. Bradford‚ M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemestry‚ 72‚ 248–254. Deschamps‚ A. M.‚ Otuk‚ G.‚ & Lebeault‚ J. M. (1983). Production of tannase and degradation of chestnut tannin by bacteria. Journal of Fermentation Technolology‚ 61‚ 55–59. Faulds‚ C. B.‚ & Williamson‚
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Food Analysis * Concerned with development of criteria of quality and identity… * Process in w/c sample is treated in a series of steps Importance: 1. It’s necessary in the dev’t and enforcement in standard of identity‚ purity and feel of processed food products. 2. It can provide studies design to improve and control the quality of natural processed food. 3. Can help in the determination of the composition of products in their normal and abnormal storage conditions. Methods
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4 unknown samples are genuine perfumes‚ and which are imposter/fake perfumes. This will be done by analysing gas chromatography plots‚ and comparing the components in the samples to that of a known standard perfume. Results: To determine which of the four sample perfumes where imposter and which were real‚ components present in each perfume were analysed using gas chromatography and compared to the known real perfume sample. The components identified for comparison included vanillin‚ 3-penten-2-one
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produce the chlorine radicals. The combination of 1-chlorobutane and sulfur chloride will produce four dichlorobutane isomers. The isomers produced and their reactivity will be analyzed by the amounts of isomers produced in the product and by gas chromatography. Procedure: 1) Assemble the apparatus in the hood using a Thermowell Heater 2) Use a 25-mL round bottom flask fitted with a reflux condenser which will be connected through a vacuum adapter to a 500-mL filter flask.
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AP Lab #5 Plant Pigments/Photosynthesis I. Identifying the Effects of Different Variables of Light and Carbon Dioxide on the Rate of Photosynthesis and Observing the Separation of Pigments Through Chromatography II. Introduction Plants have a variety of pigments‚ all of which absorb a different color of light. The three main pigments are chlorophyll a‚ chlorophyll b and carotenoids. Chlorophyll a is the primary plant pigment that absorbs red and blue light‚ which ultimately appears green to the human eye
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High Performance Liquid Chromatography Analysis of Aspirin Problem: Was aspirin (acetylsalicylic acid) successfully synthesized? Are there impurities or by-products present in the synthesized aspirin? How pure is the synthesized aspirin? Introduction: In the last experiment‚ aspirin was synthesized followed by characterization of the product using several different techniques. Melting point was a test that provided information about the identity and purity of the aspirin product. The iron(III)chloride
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time between the injection of a component into the column and the elution of that component is constant. This characteristic is used to perform qualitative or quantitative analysis. Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass‚ plastic‚ or aluminium foil‚ which is coated with a thin layer of adsorbent material‚ usually silica gel‚ aluminium oxide‚ or cellulose. A small amount of the
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different chemical properties of each compound. We will accomplish this by a separation procedure known as distillation‚ which relies on each compound having a distinct and separate boiling point. Our pure products will be analyzed with gas chromatography to determine the success of the distillation. Procedures The experiment was performed as stated in the course textbook: Pavia‚ D. L.‚ Lampman‚ G. M.‚ Kriz‚ G. S.‚ Engel‚ R. G. Introduction to Organic Laboratory Techniques: A Microscale Approach
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6 Determination of Michaelis-Menten Constant 7 3.7 Ammonium Sulphate Fractionation 7 3.8 Gel Filtration on Sephadex 7 3.9 Chromatography on DEAE-cellulose 7 4 Results 8 4.1 Freshly Prepared Crude 8 4.2 Crude Thawed after a Week of Freezing 9 4.3 Sephadex 25 Gel Filtration 11 4.4 Determination of Optimum pH 12 4.5 Ion Exchange Chromatography 13 4.6 Michaelis-Menten Kinetics 13 5 Discussion and Conclusion 14 6 5.0 Works Cited 14 7 Appendix 15 Introduction
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Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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