Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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Colouration of Silk with Natural Dyes Dr. Rajashree Phukon‚ Registration ID- AB/565 ‚ Assist. Prof.‚ Sibsagar Girls’ College‚ P.O.Sivasagar‚ Assam‚ Pin-785640‚ e-mail ID:rajashreephukon@yahoo.com and Mr Diganta Kr. Borah‚ Research Scholar‚ Department of Economics‚ Dibrugarh University‚ Assam‚ India‚ e-mail ID: digoborah@yahoo.in. ABSTRACT The study was undertaken with an aim to develop the dyeing conditions of four different natural dyes which are easily available
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Food Test 1: Test for Glucose – with Benedicts solution Benedicts solution is used to test for simple sugars‚ such as glucose. It is a clear blue solution of sodium and copper salts. In presence of simple sugars‚ the blue solution changes color to either green‚ yellow or brick-red‚ depending on the amount of sugar. Method 1) Mix smalls amount of each food sample (i.e.‚ Egg lumen‚ cylindrical piece of potato tuber‚ bread crump and crisps) in different test tubes with distilled water to make a
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Thousands of people across the world dye their hair; dating back all the way to 3400 B.C. when “people used extracts from plants or minerals that contained pigments like those of henna or black walnut shells”("History & Future Discovery of Hair Dye."). In 1932‚ hair dye was refined to create chemical reactions in the hair to change the natural structure and color of hair. Cosmetologists are near a plethora of chemicals every day that include carcinogens and mutagens they would never suspect
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SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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Skin Dye made the dye bath turn into a light yellow color; However‚ this color did not look like the color of the onion skin. The Carrot Tops Dye had not changed the color of the dyed bath but stayed clear throughout the experiment. On the other hand‚ in Picture 2‚ the Zinger tea and Chamomile tea had deeper color in their dyed baths: orange and dark red‚ respectively. None of the yarn samples were the same color as the dyed baths‚ except for the yarn sample that was dyed with the onion dye. As shown
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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HEALTH HAZARDS OF THE DYE INDUSTRY . The first step in prevention of poisoning in the dye industry includes well-ventilated‚ properly equipped buildings. Selection of workmen is important and the use of the best methods in manufacturing. These investigations‚ which are based on 2‚500 employees‚ whose blood was tested periodically‚ yield some minor diagnostic material. The hazards of the dye industry are those connected with any industry plus the poisonous chemicals necessarily handled in the
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