Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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Food Dyes A Rainbow of Risks Sarah Kobylewski‚ Ph.D. CandidateMolecular Toxicology Program University of California‚ Los Angeles and Michael F. Jacobson‚ Ph.D. Executive Director Center for Science in the Public Interest Copyright © 2010 by Center for Science in the Public Interest First Printing June 2010 Printing: 5 4 3 2 1 Summary Food dyes‚ synthesized originally from coal tar and now petroleum‚ have long been controversial. Many dyes have been banned because of their adverse
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Analysis of Food Dyes in Beverages Samantha Sequito and Anna Steenland Lab Performed: December 3‚ 2013 Lab Due: December 13‚ 2013 Introduction: Different amounts of FD&C Blue I were diluted with water to make eight differently concentrated 10 mL solutions. Samples were placed in the spectrophotometer to determine the percent transmittance of FD&C Blue 1. All of the data was summarized in graphs to predict the concentration of FD&C Blue I in a sample of Gatorade.
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Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents‚ H2O‚ Isopropyl alcohol‚ and a 2% NaCl solution‚ were introduced to the dotted chromatography strips to show three different ratios for each dye on account
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The purpose of this lab was to synthesize indigo and an azo dye and to determine how effective each dye was to dye different types of fabrics. 0.168g of 2-nitrobenzaldehyde was dissolved into acetone along with 25 drops of distilled water and NaOH to synthesize indigo. The solution was filtered‚ and the solid indigo was placed into the filter flask to reduce indigo to leucoindigo with 10mL of 3M NaOH. The flask was heated along with sodium dithionite and distilled water added to the flask. Afiber
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Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is the
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Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
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In this Laboratory experiment‚ my lab partner Alexander and I were able to understand thoroughly the physical and chemical properties of salt (NaCl) and sand (SiO2). Followed by the right procedure we were able to design and test out the components of both NaCl and SiO2 in order to separate the unknown mixture that we were able to find out about. We were also given the task to provide the percent composition of the mixtures. Therefore‚ the separation of components among this experiment allowed us
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Information and Research 1. Chromatography is an analytical methor or technique that serves mainly as a tool for the examination‚ separating and identifying mixtures of chemical substances that are or can be coloured. 2.check the presence of any contamination in the manufactured compounds h as medicine‚ Contaminants in rainwater Analysis of narcotics Detection of substances in urine http://en.wikipedia.org/wiki/Paper_chromatography http://www.chemguide.co.uk/analysis/chromatography/paper.html http://chem
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Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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