SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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Skin Dye made the dye bath turn into a light yellow color; However‚ this color did not look like the color of the onion skin. The Carrot Tops Dye had not changed the color of the dyed bath but stayed clear throughout the experiment. On the other hand‚ in Picture 2‚ the Zinger tea and Chamomile tea had deeper color in their dyed baths: orange and dark red‚ respectively. None of the yarn samples were the same color as the dyed baths‚ except for the yarn sample that was dyed with the onion dye. As shown
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
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HEALTH HAZARDS OF THE DYE INDUSTRY . The first step in prevention of poisoning in the dye industry includes well-ventilated‚ properly equipped buildings. Selection of workmen is important and the use of the best methods in manufacturing. These investigations‚ which are based on 2‚500 employees‚ whose blood was tested periodically‚ yield some minor diagnostic material. The hazards of the dye industry are those connected with any industry plus the poisonous chemicals necessarily handled in the
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Component seeks to separate‚ identify‚ quantify and analyze the active component found in Biogesic®‚ which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore‚ this study applies thin layer chromatography (TLC) technique since it is the simplest‚ cheapest yet most effective approach in terms to the study’s objectives. The TLC method implies Rf (Rate of flow) values where it is given by the distance from origin to center of subtance spot over the
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Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
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9.1 Orbitals and Theories of Chemical Bonding 1. Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct? a) MO theory predicts that electrons are localized between pairs of atoms. b) In VB theory‚ bonding electrons are delocalized over the molecule. c) MO theory accurately describes bonding in O2 and NO‚ VB theory does not. d) VB theory can describe molecular bonding in excited states. e) MO theory is used to accurately predict
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The purpose of this lab was to use gas chromatography to determine the relative amounts of the three components of gas products from the dehydration reaction of 1- and 2-Butanol with sulfuric acid and the dehydrobromination reaction of 1- and 2-Bromobutane with potassium tert-butoxide. E1 reactions are two-step unimolecular reactions. The first step of an E1 reaction occurs when a leaving group‚ which consists of a weak base‚ leaves an organic compound‚ leaving an intermediate carbocation. In the
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