OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and determining them by using the Rf values obtained from the paper chromatography technique. The hypothesis of the experiment was that all of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments used were a 18 x 150 mm test tube with stopper‚ graduated cylinders‚ Erlenmeyer flask‚ mortar and pestle‚ metric ruler‚ tall jar‚ acetone‚ tiny
Premium Thin layer chromatography Chlorophyll Laboratory glassware
spp and its application in dye decolorization Project supervisor: Dr. R. Masalu Lab scientist: Mr. Chuwa INTRODUCTION: Due to rapid industrialization and urbanization‚ a lot of chemicals including dyes are manufactured and used in day-to-day life. Dyes are synthetic and aromatic molecular structural compounds. According to their dissociation in an aqueous solution‚ dyes can be classified as acid‚ direct reactive dyes (anionic)‚ basic dyes (cationic) and disperse dyes (nonionic). They are used
Premium Dye Enzyme
EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
Free Chromatography Thin layer chromatography
Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
Free Chromatography
How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
Premium Chromatography Human Gas chromatography
Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture‚ without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy‚ chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words‚ "chroma" meaning color
Free Chromatography Thin layer chromatography
Visual Arts Topic: Textile and Design Manipulation Topic: Tie Dye Time: 4 sessions x 30 mins each General Objective To be able to use appropriate materials to create designs on fabric by various tie dyeing techniques. Specific Objectives At the end of the lesson students should be able to: ✓ 1. Define Tie Dye ✓ 2. List materials needed for tie dyeing ✓ 3. Explain Tie Dye as a resist dyeing ✓ 4. Know different techniques and explain each of
Premium Dye Color Lesson plan
answer through your investigations: 1. Which fabric (a) cotton‚ a natural fiber and (b) rayon‚ a synthetic fiber is best dyed with mayana-alum dye combination? 2. Often‚ mordants are added to fix the dye onto the fabrics. These are usually inorganic oxides or salts which form colored complex with the dye. Which mordant is more suited for dyeing cotton with dye extracted from eggplant skins? 3. Which mordant is more suitable for dyeing a synthetic fiber with the synthetic extract from mangosteen
Premium Dye Fiber Alum
Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the
Premium Chromatography Analytical chemistry Gas chromatography
Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also
Premium Thin layer chromatography Chromatography Solubility