ABSTRACT Rising energy prices and environmental problems led to the increase in alcohol demands in many aspects‚ like in making fuels. Many people are doing researches about making their own product so as to lessen their everyday expenses. Right now‚ Filipino scientists are in think of the search for alternative fuels‚ with the experts from the University of the Philippines in both Diliman and Los Banos campuses searching all over the archipelago for energy sources from the forests to the bottom
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Separation and Identification of Plant Pigments by Paper Chromatography Biology 1107L Introduction All living organisms require energy for their chemical processes. The ultimate source of this energy is the sun. Plants convert light energy into the chemical energy of sugars. During photosynthesis pigments are used to capture light energy. Pigments of green plants can easily be separated and identified using a technique called paper chromatography. The purpose of this experiment
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and thin layer chromatography of plant pigments CHEMISTRY 201L EXPT 04 PAGE 01 - 12 Noel Angelo P. Kalacas*‚ Hanna Mae Laluces‚ Ina Bianca Lanuza Department of Chemistry‚ College of Science *Corresponding author; e-mail: knight_BeNcH66@yahoo.com Abstract Chromatography is a powerful technique for separating and/or identifying the components in a mixture. There are different types of chromatography and each has its own strengths and weaknesses. In this experiment‚ pigments of the chili
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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Spinach Leaves The goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a‚ chlorophyll b‚ carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the data was graphed it clearly showed the higher rate of red light absorption over green light. These
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spectrum) by James Clerk Maxwell; this included: cosmic rays‚ visible light‚ x-rays‚ and radio waves. For plants to use sunlight‚ they have to absorb it. So pigments absorb light energy. Chlorophyll is a pigment in leaves that reflect green light waves. This is the most important pigment in photosynthesis and there are also accessory pigments used in plants. Also in this lab there is the internal and external anatomy of a typical leaf. Materials and methods: The first procedure dealt with the
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extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon adding a solvent mixture of hexane and acetone‚ the chlorophyll and carotene pigments can be
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Introduction The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis‚ based on starch production‚ which wavelengths of light are involved in photosynthesis‚ and identify plant pigments found in a plant leaf by means of paper chromatography. Life on Earth is dependent entirely on the energy from the Sun‚ not only to keep the planet at a suitable temperature but also to provide the energy required to sustain life. The energy of the Sun‚ in the form of
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Question 1: You have carried out an investigation into the pigments that make up chlorophyll. Use the chromatogram you obtained when carrying out this investigation. Clearly explain the separation technique of paper chromatography in terms of partition. Calculate the Rf value of one of the constituent pigments and compare it to the book value. A paper chromatography was used to separate the pigments that make up chlorophyll to analyse‚ identify‚ and quantify the components‚ using paper (stationary
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Chromatography Lab Questions Pre-Lab Questions 1. A pencil is used rather than a pen to mark the “starting line” in step two because a pen or marker while be used to place a small dot of ink in the center of the X that is made with a pencil. 2. Capillary action is filling of pores in a solid with a liquid. Capillary action is caused by spotting or absorption and is allowed to seep or flow through. 3. The type of pen that will probably give better separation in this paper chromatography
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