5.5 Candy Chromatography Background Information: Paper Chromatography is a separation tool in which pigment is put on a paper made of cellulose and water‚ and placed in a solvent‚ in this case isopropyl alcohol. Due to capillary action‚ the solvent crawls up the paper‚ separating the pigments. This technique is used to identify components of a mixture‚ even unknown ones‚ and can be used to isolate components into pure samples. Real world uses of this technique includes identifying certain biomolecules
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Hypothesis. If pigment from euonymus alatus (burning bush) leaves is extracted‚ then carotenes and xanthophylls will appear when a solvent‚ made of 9 parts petroleum ether and 1 part acetone‚ goes up Whatman #1 filter paper Purpose. The purpose of this experiment was to demonstrate the presence of carotenes‚ xanthophylls‚ and chlorophylls in leaves. Methods and Materials. A piece of Whatman #1 filter paper was cut to fit into an ignition tube. One end was cut to form a V‚ and the sides of
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NOTES AND COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error
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CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
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different types of soda with different color. Is soda really made of natural color from fruit? Is that safe to drink? Will that kill you? So there is science behind this. The colors of the soda is made of pigments. The fruit color will make the color of the soda brighter. Column Chromatography is a new technical method to separate the chemical inside the mixture. I can use this method to separate the dyes of the grape soda. The chemical mixtures in the grape soda will pour through the column and
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Outline: Title The title of the lab should be at the top of the lab report. Background Information and Research 1. Give a simple explanation‚ in your own words‚ of what paper chromatography is and what it is used for. 2. Give at least three real-world uses for paper chromatography used in the fields of chemistry and biology. 3. Be sure to include references for any research conducted for this section. Purpose In one or two complete sentences‚ state the purpose of this laboratory
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Background: Paper chromatography is one method for testing the purity of compounds and identifying substances. Paper chromatography is a useful technique because it is relatively quick and requires small quantities of material. Safety: Warnings: * Rubbing alcohol (or isopropyl alcohol) is flammable‚ so be sure to keep it away from high heat or heat sources. * Keep pigments and solutions away from your eyes‚ wash your hands carefully after coming in contact with any solutions or pigments‚ and clean
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Lab 5 Column Chromatography: Isolation of Lycopene from Tomato Paste Reading: Zubrick‚ pages 79-82‚ 127-130‚ 138-139‚ 141-143‚ and 235-240 Pre-lab: look up the structure of lycopene. Introduction: Lycopene is the red pigment in ripe tomatoes and‚ as an antioxidant‚ helps to fight certain cancers. In this lab you will isolate lycopene from tomato paste. To do this you will first extract carotenoid pigments from the paste and then use column chromatography to isolate the lycopene from
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Photosynthesis Homework April 8‚ 2013 Reviewing Your Knowledge 2. A pigment solution contains compound A with 4 polar groups and compound B with 2 polar groups. You plan to separate these compounds using paper chromatography with a non-polar solvent. Predict the location of the two bands relative to the solvent front. Explain your answer. Since compound A is probably more polar than compound B‚ compound B will move further in the chromatography. Since the solvent is non-polar‚ the less polar compound will
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Journal of the American Chemical Society 63: 877-879. Sterling‚ T.‚ Ph.D. 2004. Transpiration-Water Movement Through Plants. Zafaralla‚ M.T.‚ et.al. 2007. Explorations in Plant Science. pp. 63-66. Henderson State University. Separation of Pigments by Paper Chromatography. Retrieved from: http://www.hsu.edu/pictures.aspx?id=1653
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