Mario Renteria Mrs. Weathers Chem 04a 27 Jan‚ 2012 Lab Report Introduction: A popular technique in separating components of a mixture is paper chromatography. We use paper chromatography to separate mixtures and isolate there components to collect them individually. In this experiment‚ we will use acid- base indicators to help determine the unknown mixture. Acid- base indicators will change in color when the presence of pHs rise and fall when acids or bases are added to a solution. After setting
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validation for paracetamol drug includes the parameters such as accuracy‚ precision‚ selectivity‚ sensitivity‚ reproducibility and sensitivity. All this validation methods are applicable to bio-analytical methods such as Gas Chromatography and High Performance Liquid Chromatography. Unfortunately the most undesirable feature of drugs containing the DHP (dihydropyridine) group is high sensitivity to light .Such drugs are regarded as photo labile. Therefore the photo stability testing has become an important
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1. In table format‚ provide the Rf values you recorded for each of the 3 compounds in Part I. List compound on one axis of your table and solvent system on the other. If multiple spots were present for a compound‚ give Rf value for each component and state if the component was major or minor. | 100% hexane | 25% EtOAc in hexane | 10% EtOAc in hexane | Fluorene | 0.34 cm | 0.94 cm | 0.67 cm | 9 - fluorenol | 0.00 cm | 0.49 cm | 0.05 cm | 9 - fluorenone | 0.00 cm | 0.69 cm | 0.21 cm |
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/50 Lab 9 Report Template: Acid Catalyzed Dehydration of 2-Methylcyclohexanol Abstract In Experiment 9‚ distillation was used to carry out the dehydration of 2-methylcyclohexanol using 4:1 mixture of phosphoric and sulfuric acid. Gas Chromatography was then used to measure the products. Two products were formed. The theoretical yield of the alkene mixture was 3.914 g. The actual yield of the mixture was 0.73 g. The percent yield for the reaction was 18.65%. Peak A had an area of 0.3 mm2
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of the GFP protein. This indicates that the molecule responsible for the observed green trait was protein. The transformation efficiency was found to be 793.75 transformants per microgram. The protein was filtered out of the cell and column chromatography was used as a means to isolate the protein by taking advantage of its hydrophobic properties. This was performed by washing the column containing the GFP protein amongst other proteins with buffers varying in salt concentrations. As expected
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melting point*Capillary tube *Tesco aspirin*Melting apparatus * synthesised aspirin | Apparatus for chromatography Materials for chromatography *Chromatography tank *Ethanol dichloromethane *Capillary tubes * Tesco pure aspirin *Weighing boat
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(Author’s name) (Professor’s name) (Course details) (Date Abstract The aim of the lab was to separate and analyse analgesic drugs in a drug tablet. The method used to separate the components was Thin Layer Chromatography (TLC) with silica adsorbent as the stationary phase and 0.5% glacial acetic as the mobile phase. In one plate‚ five known samples were used as the reference‚ that is: Aspirin; Caffeine; Ibuprofen; and Salicylamide. Aspirin and Salicylamide were the only samples that fluoresced
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than water) and will bond to the H+ ions. This resulted in a bright yellow mixture. Anhydrous CaSO4 is used as a drying agent. It absorbs all of the H2O in the mixture. If H2O is present‚ then the product cannot be analyzed by gas chromatography. Gas chromatography is used to determine the amount of
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[PARVATIBAI CHOWGULE COLLEGE(H.S.S)-MARGAO-GOA] Chemistry chart Std-XII Name______________________________________________ Class____________________________________ Div__________________ Roll No___________ Batch No_______ 1 [PARVATIBAI CHOWGULE COLLEGE(H.S.S)-MARGAO-GOA] Chemistry chart Std-XII VOLUMETRIC ANALYSIS REDOX TITRATIONS Experiment No:- 1 Date:--------------- Aim:- You are provided with two solutions as follows. Container A: -----------------M/N Stock Solution of Hydrated
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physical properties. Paper chromatography will be used to separate the dye colors from M&M candy to see which colors contain yellow #5. Gravimetric separation will be used to separate salt and sand from a mixture. Procedure The procedure for this lab can be found on pages 16 – 30 in “General Chemistry CHE110L Laboratory Manual Fall 2013”. Data Tables Carbon copies of data tables have been attached to the back of this lap report. Calculations Paper Chromatography- 1. Yellow M&M’s Rf Value:
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