Column Chromatography of Plant Pigments Jaybee Balilea‚ Sharmaine Baysic‚ Maria Anjelette Patricia Belen 3Bio-7‚ Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use
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EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
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& Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation. • Use evidence
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Lab 11- Plant pigment and Photosynthesis Zelda Salfati December 20th‚ 2012 BIOL 1012Y Partners Lab: Nicole Mintz‚ Bayla Kronman
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
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Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also
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Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture‚ without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy‚ chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words‚ "chroma" meaning color
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How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
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Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the
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