Yuri Anthony D. Sucgang BMLS-IA Electrophoresis Machine Gel Electrophoresis Apparatus(Aragose Gel) Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. Gel electrophoresis is a laboratory procedure used to separate biological molecules with an electrical current. In this lesson‚ we’ll review how agarose gel electrophoresis works and introduce the equipment necessary to perform an electrophoresis experiment.
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IDENTIFICATION OF UNKNOWN SOLUTIONS USING TLC CHROMATOGRAPHY Purpose: To become familiar with the principles and terminology of TLC chromatography to identify an unknown molecule based on comparisons with known laboratory standards and Rf factors. Background Thin Layer Chromatography: Chromatography is a sophisticated method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that
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Lab #1 Extraction and Thin Layer Chromatography Kaya Gaudet 6784928 January 9‚ 2013 Line Structure References BenzophenoneBiphenylBenzoic Acid Extraction: A process used to separate different compounds in a mixture based on their solubility in an immiscible substance. Usually an aqueous and organic phase are used. During extraction the desired compound moves one phase and leaves the unwanted substance behind. Extraction normally has to be done a few times to leave all of the impurities
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Briefing Thin-layer chromatography (TLC) is an extremely valuable analytical technique. It provides a rapid separation of compounds‚ and gives an indication of the number and nature of the components of a mixture. TLC can also be used to identify compounds by comparison with known samples‚ to check the purity of a compound‚ or to monitor the progress of a reaction‚ an extraction‚ or a purification procedure. This experiment will introduce you to the mechanics of TLC‚ and the chemical principles
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distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
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CH3I Before coming to lab‚ please review the following techniques: "Reluxing a reaction‚" "Extraction and washing‚" "Drying an Organic Solvent‚" "Evaporating an Organic Solvent‚" "Analyzing a Solution by Thin Layer Chromatography (TLC)‚" "Separating Mixtures by Column Chromatography‚" and "Characterizing Compounds by IR." Notice that this lab brings together most of the techniques that we have used this semester. Then complete the following table before coming to class so that you will be prepared
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January 27‚ 2014 Introduction This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second‚ each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen‚ Aspirin and Caffeine. Finally‚ calculating the Rf value by measuring the mobile and stationary phase. Chromatography is a technique used to separate mixtures into their compounds. It’s based on a mobile phase and a stationary
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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Proteins (ACTIVITY 8) Gallemit‚ Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and
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Introduction: The purpose of this experiment is to use sodium borohydride to reduce benzil. However‚ stereochemistry allows for five possible products. If only one carbonyl group is reduced during the reaction a racemic mixture of benzoin will be the product that is produced. After the first reduction a chiral center forms causing the second reduction to occur from only one side of the ketone. Depending on which side the second reduction take place there are three possible products including: a racemic
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