Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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Post Lab #4- Column Chromatography Organic Chem 3418-2 March 3‚ 2011 Theoretical Background- The fluorene and fluorenone mixture was separated by first dissolving the mixture in heptane. Since “like dissolves like”‚ fluorene dissolves with the non-polar heptane and the polar fluorenone dissolves in the polar ethyl acetate solvent. This phenomenon was illustrated in class before the experiment‚ when it was pointed out why water will not dissolve fluorene‚ fluorenone‚ or transstilbene
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Bangabandhu Sheikh Mujibur Rahman Science and Technology University‚ Gopalganj. Title : Assignment on HPLC (High-performance /High-Pressure Liquid Chromatography) Introduction: HPLC is an advanced form of liquid chromatography used in separating the complex mixture of molecules encountered in chemical and biological systems‚ in order to recognize better the role of individual molecules by using significantly higher pressure (50–350 bar). Compounds are separated by injecting a sample
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RADICAL HALOGENATION AND GAS CHROMATOGRAPHY Abstract In radical halogenations lab 1-chlorobutane and 5% sodium hypochlorite solution was mixed in a vial and put through tests to give a product that can then be analyzed using gas chromatography. This experiment was performed to show how a radical hydrogenation reaction works with alkanes. Four isomers were attained and then relative reactivity rate was calculated. 1‚1-dichlorobutane had 2.5% per Hydrogen; 1‚2-dichlorobutane had 10%; 1‚3-dichlorobutane
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Division of Environmental Epidemiology University Utrecht 05/06/2007 Biopharmaceutical Technology Europe Volume 21‚ Issue 10 Valencio Salema‚ Lalit Saxena‚ Priyabrata Pattnaik ‘Removing endotoxin from biopharmaceutical solutions’ 01/09/2009 ‘Ion Chromatography: A New Technique for Clinical Chemistry’ Courtney Anderson Vol. 22‚ No. 9 1976 Biochemistry Jeremy M. Berg‚ John L. Tymoczko‚ Lubert Stryer 2006 ‘Curtin’s industrial biotechnology research’ (http://www.theborneopost.com/2013/02/12/curtins-industrial-biotechnology-research/)
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Contents Title Page No. 1.0 Introduction 1 2.0 Sample Treatment 3 3.0 Extraction 4 4.1 Solid-Phase Extraction 4.0 Separation 6 5.2 High Performance Liquid Chromatography (HPLC) 5.3 Thin Layer Chromatography (TLC) 5.0 Detection and Identification 7 6.4 UV-Vis Spectrophotometry 6.5 Mass Spectrometry 6.6 Nuclear Magnetic Resonance Spectrometry 6.0 Health Benefits of Daidzein 18
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Limited‚ Bangalore College supervisor: Dr: T.Yasodha‚ Head- Department of Biotechnology‚ Shri Andal Alagar College of Engineering‚ Mamandur‚ Tamilnadu‚ India Objective: Purifying microbe derived therapeutics from fermentation broth by various chromatography and analysis using RP HPLC techniques- specializations on lipstatin drug. Abstract: The main focus area of this project is to purify the microbe derived drug lipstatin by using various trials of chromatographic columns with different packing
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HPLC Theory: System Suitability Parameters High performance liquid chromatography is defined as a separation of mixtures of compounds due to differences in their distribution equilibrium between two phases‚ the stationary phase packed inside columns and the mobile phase‚ delivered through the columns by high pressure pumps. Components whose distribution into the stationary phase is higher‚ are retained longer‚ and get separated from those with lower distribution into the stationary phase. The
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INTERSCIENCE‚ B.V.: SEEKING A NEW MARKET FOR GAS CHROMATOGRAPH| B2B Marketing - Case Analysis | Submited To: Prof. S. Garimella Dated: 18th July 2010 Submitted by: Aditi Sharma Amandeep Singh Ashwini Sinha Divay Makkad Prashant Akhawat IMI | INTERSCIENCE‚ B.V.: SEEKING A NEW MARKET FOR GAS CHROMATOGRAPH | Contents 1. Introduction 3 2. Markets for gas chromatograph 3 3. Players in the contract laboratory market and the price war 4 4. Business Model of Agilent
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Caffeine is a drug that is used to improve mental alertness by stimulating the central nervous system [1]. It is an ingredient found in a various amount of products sold on the market today‚ such as coffee‚ tea‚ chocolate‚ soft drinks‚ and energy drinks. Caffeine can be taken by mouth or intravenously (by IV) to help alleviate breathing problems in infants and can even be used in combination with some pain killers to treat migraines after epidural anesthesia. Moreover‚ it can be helpful in preventing
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