The average University student has many tasks to accomplish and much studying to do while combating the onslaught of sleep. Students at the University of the West Indies Mona are no different and recommended amongst themselves a highly rated and popular health supplement “Yeast-Vite” [8]. Yeast-Vite is a health supplement pill which helps people fight fatigue and improve alertness. The active ingredients in Yeast-Vite are caffeine‚ vitamin B1‚ vitamin B2‚ vitamin B3. The other ingredients are:
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the Kool-Aid was correct. However‚ solely based upon the Rf values‚ the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However‚ the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because one color band was of a blue tint and the other‚ a yellow tint. Therefore‚ based on this qualitative data‚ the dyes in the green Kool-Aid are
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Maithani M et al. / Pharmacie Globale (IJCP) 2010‚ 5 (05) Available online at www.pharmacie-globale.info ISSN 0976-8157 Research Article PHARMACIE GLOBALE INTERNATIONAL JOURNAL OF COMPREHENSIVE PHARMACY DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE DETERMINATION OF CHLORPHENIRAMINE MALEATE AND PHENYLEPHRINE IN PHARMACEUTICAL DOSAGE FORM Mukesh Maithani*‚ Richa Raturi‚ Vertika Gautam‚ Dharmendra Kumar‚ Amrendra Kumar Chaudhary‚ Anand Gaurav and Ranjit Singh School of
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Experiment # 4 Paper Chromatography 1. Why is the chromatogram developed in an essentially closed system? - The chromatogram is developed in a closed system in order to prevent the solvent to evaporate. Most solvents used in the chromatograph are toxic and flammable. It is also put in a close system to reduce the chance of outside factors affect the chromatograph. 2. What is the main advantage of 2-dimensional paper chromatography over a 1-dimensional one? - The
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filtration is a non-adsorptive chromatography technique that separates molecules on the basis of molecular size. Desalting and buffer exchange are two special examples of gel filtration that are widely used in many downstream bioprocesses. Desalting is used to completely remove or lower the concentration of salt or other low molecular weight components in the sample while buffer exchange replaces the sample buffer with a new buffer. Gel filtration is one of the easiest chromatography methods to perform because
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Student Number: xxx Class: xxx Aim The aim of this experiment was to determine the concentration of aspartame in a sample of diet Coke® using HPLC as investigation tool. Introduction High Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques of nonvolatile organic compounds. Chromatographic processes can be described generally as mass-transfer between stationary and mobile phase. In HPLC‚ a liquid mobile phase is used to
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due to the structure of disperse red 9 being more symmetrical than that of disperse blue 3 and having more nonpolar bonds. Disperse blue 3 is more polar because it has a hydroxide bond and has a larger dipole. The principle behind using column chromatography is that it separates compounds based on polarity. The alumina serves to allow for a purer separation than TLC plates because it has a more polar surface than silica gel does. The less polar dye moves first because it is not as soluble in the stationary
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Natural Products Experiment Niki Robinson 10008562 Practical 2: Identification and quantification of taxifolin from milk thistle Aim: to Identify and quantify the amount of taxifolin from three samples Silymarin stock solution‚ taxifolin and silybum marianum extract. ` Methodology: Using auto-pipette (20-100 microlitre)‚ pipetted 0.06 mg of Taxifolin was added to 10ml conical flask and made up to the mark (10ml) with H2O.This procedure was repeated with the same amounts foe silymarin stock
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1.5.2.5 Detectors The detectors used in UPLC should be able to handle very fast scanning methods because half-height peak widths of less than one second are usually obtained with columns packed with 1.7 µm particles. The detector must be able to give high sampling rate adequate to capture enough data points across the peak for an accurate and reproducible integration of analyte peak. The dispersion (volume) of the detector flow cell must be minimal to maintain separation efficiency. Conceptually
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