Lab 4: Photosynthesis and Chromatography of Spinach Leaves Discussion: The chromatography of the spinach leaves was taken to establish what pigments were present in the chloroplasts of the leaves. The solvent carried and separated the different pigments up the cellulose paper. The solvent moved up the paper because of capillary action because of adhesion of the solvent molecules and the paper. The pigments dissolved in the solvent also were carried up the paper and were separated because
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Separation and Identification of Organics from an Aqueous/Organic Environment through Physical Manipulation Date Lab Performed: Nov. 11/2013 Date Lab Submitted: Nov. 18/2013 Introduction We conducted this experiment to investigate the technique of liquid / liquid extraction‚ the miscibility of organics and water and the effect of a mixture of compounds in methylene chloride versus pure compounds retention time as introduced to a Gas
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1. What physical evidence did you find at the victim’s home? I found a wallet‚ possible flammable liquid‚ beer bottles. 2. Describe the process that you used to take a sample of the puddle-shaped burn pattern on the floor. Why did you also take samples from the portion of the floor that was not burned? I scraped up the material from the puddle-shaped burn‚ and then used tweezers to store it. I took samples from the portion of the floor that wasn’t burned to compare it to the puddle-shaped burn
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What physical evidence did you find at the victim’s home? Matches Space heater Bear bottles The girlfriends wallet Describe the process that you used to take a sample of the puddle-shaped burn pattern on the floor. Why did you also take samples from the portion of the floor that was not burned? To take a sample of the burned floor we scraped the burnt wood will we got loose pieces of it. We did the same to take a sample of the wood that had not been burned. We took a sample of the burned
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1. What physical evidence did you find at the victim’s home? Matches‚ Natalie’s ID‚ burned floor‚ and space heater. 2. Describe the process that you used to take a sample of the puddle-shaped burn pattern on the floor. Why did you also take samples from the portion of the floor that was not burned? I used a chisel to scrape up some wood and then used tweezers to pick it up and put it in an airtight container. I would take samples from none burned wood to compare the results in testing. 3. What
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he v-shaped pattern often occurs in the area where a fire originated‚ so it is possible that the space heater was the fire of origin (tests needs to be run to confirm). The crazed glass (by lab results) indicated that crazing is the result of rapid cooling of hot glass by the application of water. The protected surface shows there was an object on the floor protecting the area‚ What physical evidence did you find at the victim’s home? Describe the process that you used to take a sample of the puddle-shaped
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Introduction Flash-Column Chromatography is another useful technique used to separate and purify complex mixtures of compounds. In this experiment‚ TLC is used to determine the best solvent system for the column. Due to Flash-Column Chromatography1 being an inexpensive technique‚ many scientist use this process for drug and natural product purification. Method By using the information from Experiment 6B‚ the TLC solvent system used was 30% EtOAc: 70% Hex. Experiment 7 was broken into two parts‚
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Identification of Plant Pigments by Paper Chromatography Biology 1107L Introduction All living organisms require energy for their chemical processes. The ultimate source of this energy is the sun. Plants convert light energy into the chemical energy of sugars. During photosynthesis pigments are used to capture light energy. Pigments of green plants can easily be separated and identified using a technique called paper chromatography. The purpose of this experiment is to separate
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by Laboratory Reagents‚ Legacy Product Code: S/0380/48‚ CAS Number:8047-15-2) were powdered and analyzed as potassium bromide (KBr) pellets using FTIR (Model-JASCO FT/IR 4100 LE‚ made in Japan; Range: 4000-400 cm-1) Prabhu et al. (2013). Gas Chromatography–Mass Spectrometry (GC–MS) analysis: Acid hydrolysis of saponin was carried out according to the method described by Metwally et al. (2012) with some modification. Saponin was hydrolyzed with 2 N HCL (6 hr at 100 o C) under reflux‚ the residue
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liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together with a standard caffeine. The Rf value obtained for both isolated caffeine and standard caffeine was 0.24. Keywords: solid-liquid extraction‚ multiple liquid-liquid extraction‚ sublimation‚ thin layer chromatography‚ percent caffeine Introduction Many organic compounds are obtained from natural sources through isolation. In this experiment
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