Partners Lab: Nicole Mintz‚ Bayla Kronman and Dvora Feinblum ABSTRACT This experiment will enable us to separate the pigments of a plant by using the method of paper chromatography. Thanks to this technique the pigments were painted on the strip of paper‚ and we were able to calculate the distance each of them went up: the Rf value. We saw that carotene went the farther up and that chlorophyll ’a’ stayed next to the bottom
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Biochemistry Exam I Terminology Boiling point is the temperature at which the vapor pressure of the liquid equals the pressure surrounding the liquid. At this temperature the liquid phase changes into the gaseous phase (also a colligative property) Melting point is the temperature at which the solid and liquid phases are in equilibrium. At this temperature the solid state changes into a liquid phase (also a colligative property) Specific heat is the amount of energy (in joules or calories) needed
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and Phenylalanine (not in sequence). We compare the Rf values obtained from unknown and compare it with the standards. The amino acid identity is the one that has the lowest absolute value of difference with the unknown. In using the thin layer chromatography in the experiment‚ Sanger’s method was also incorporated to determine the proper sequence of the dipeptide. It is a colorimetric method that uses 1- fluoro- 2‚4- dinitrobenzene (FDNB) and reacts with free amino ‚ imidazole and phenolic groups
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To separate acetaminophen‚ aspirin and caffeine from painkilling drugs. 2.To determine the melting points of acetaminophen‚ aspirin and caffeine 3.To identify the separated components(acetaminophen‚ aspirin and caffeine) by thin-layer chromatography Apparatus and Reagents Used : 1. 4:1 (v/v) 1-Butyl ethanoate/ethanoic acid 2. 3M Sodium hydroxide solution 3. 3M Hydrochloric acid 4. Iodine 5. Dichloromethane 6. Ethanol 7. Anhydrous MgSO4 8. Mortar and pestle 9. Beakers 10. Capillary
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An electrophilic aromatic substitution reaction was performed on bromobenzene with nitric acid producing both 2-bromonitrobenzene and 4-bromonitrobenzene. Products of the reaction were purified through multiple recrystallizations and column chromatography creating multiple crops of a yellow powder. The percent yield of products was determined to be 51%. The melting point of Crop 1 was found to be 110-115 °C‚ and Crop 2 was found to be 37-90 °C indicating impurity in both crops. TLC analysis shows
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An audit of these strategies is given in the writing (39). CE is an electrophoretic strategy that is often lumped with chromatographic routines in light of the fact that it shares a considerable lot of the basic necessities of chromatography. A wide scope of mixes can be determined utilizing TLC by utilizing diverse plates and versatile stages. GC is a helpful strategy for measurement. It can give the coveted determination‚ selectivity‚ and simplicity of evaluation. This strategy
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so that it is safe for drinking. Mixtures contain two or more substances that are not in a fixed ratio‚ and while some mixtures‚ such as concrete‚ are useful‚ others including salt water‚ are not as useful to us. Filtration‚ distillation‚ paper chromatography‚ and centrifugation are just a few methods used for separating mixtures. There are many methods of separating mixtures. A more commonly known and used method is filtration. Filtration separates mixtures using filter paper‚
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solids and liquids. LEARNING OBJECTIVES Students should be able to: ➢ Define the key terms such as solution‚ solvent‚ solute‚ filtrate‚ residue‚ immiscible and miscible liquids‚ distillate‚ sublimation‚ melting and boiling points and chromatography. ➢ Use melting and boiling points to differentiate between pure and impure substances. ➢ Select appropriate methods for separating given mixtures. ➢ Describe appropriate methods of separating given mixtures. ➢ Perform simple
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|Lesson one – Atomic structure and mass | |1. How to represent atoms. |Starter – what is an atom? What do we call the particles that make up an |Can represent atoms showing atomic number |Model of an atom. |Atomic mass worksheet | |2. The relative masses of protons‚
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risk the possibility of death when their blood alcohol content ranges from 0.400% to 0.500%‚ or exceeds 0.500%. Qualitative and quantitative analysis of ethyl alcohol in postmortem specimens are commonly explored with the use of gas chromatography. Gas chromatography can assist in determining the amount of ethyl alcohol present at the time of death. This information could reveal whether a high level of ethyl alcohol contributed to the cause of death.
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