Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent
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Matter Lab Perform Paper Chromatography Lab Turn-in: 1) Changes: Physical or Chemical Lab - Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 2) Special Physical Properties of Matter Lab – Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 3) Paper Chromatography Lab – Completed Procedure / Observations
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such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate‚ initial protein concentration was shown to be 100 mg/ml. The final protein concentration of the pooled affinity sample
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extraction with 10 mM formic acid solution and precipitation at low temperature by centrifugation. The utilise of C-18 reversed-phase columns were slightly more polar performed to achieve a better separation of acrylamide. Eluent system of liquid chromatography used a time programme gradient in order to obtain a better selectivity. The mobile phase was a mixture of 10 mM formic acid and methanol. The performance parameters of precision‚ accuracy and recovery were conducted to assure that the analytical
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The five common methods that can be used to separate various types of mixtures are filtration‚ fractional distillation‚ evaporation‚ sublimation‚ and chromatography. Evaporation is the changing of a liquid into a gas often under the influence of heat. A mixture of a solid and liquid can be separated by evaporation. Filtration is a technique that uses a porous barrier to separate a solid from a liquid. A liquid
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Mixtures & Solutions Lab Report By Nathan Mitchell 4/23/15 Background Information & Research 1. Paper Chromatography is a method used for the separation of colors which are also referred to as colored chemicals/substances or pigments. This method is used for experiments‚ to identify coloring agents and to separate out a compound into its various components. 2. 3 real-world uses for paper chromatography include forensic testing‚ performance enhancing drug testing‚ and Ebola immunization. 3. References:
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05.05 Mixtures and Solutions Background: Paper chromatography is one method for testing the purity of compounds and identifying substances. Paper chromatography is a useful technique because it is relatively quick and requires small quantities of material. Safety: Warnings: * Rubbing alcohol (or isopropyl alcohol) is flammable‚ so be sure to keep it away from high heat or heat sources. * Keep pigments and solutions away from your eyes‚ wash your hands carefully after coming in contact
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Colorado Northwestern Community College Science of Biology Mrs. Farrow Lab 3 – Slime Time Submitted by Chase Kenemer 22 February 2015 Abstract Polar solvents dissolve‚ or pick-up‚ polar substances and non-polar solvents dissolve‚ or pick-up‚ non-polar substances. In the conducted experiment‚ the polarity of molecules and their properties are explored. The results of using two solvents on both polar and non-polar inks‚ further verify this to be true. The student conducted the experiment
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solution contains compound A with 4 polar groups and compound B with 2 polar groups. You plan to separate these compounds using paper chromatography with a non-polar solvent. Predict the location of the two bands relative to the solvent front. Explain your answer. Since compound A is probably more polar than compound B‚ compound B will move further in the chromatography. Since the solvent is non-polar‚ the less polar compound will be more soluble in the solvent than the more polar one. Applying Your
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The E2 reaction performed began by dehydrohalogenating the 3-chloro-3‚ 7-dimethyloctane with potassium hydroxide in aqueous ethanol. The 3-chloro-3‚ 7-dimethyloctane will lose a hydrogen from three different carbons that cause the formation of three constitutional isomers. If the hydroxide ion attacks a hydrogen on the 3-methyl carbon‚ then 2-ethyl-6-methyl-1-heptene will be the product. If a hydrogen on the carbon-2 was attacked‚ then the product would be 3‚ 7-dimethyl-2-octene. If a hydrogen was
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