Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally Affinity Chromatography on a Cibacron blue Sepharose stationary
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Why Conclusion is Important in Term Papers Writing Conclusion is the last part of any type of paper‚ usually a college term paper comprised of three main parts introduction‚ body and finally conclusion. The conclusion of a term paper should be well written because as it is the last part of a paper so it should have a lasting impression on readers and audience. It is an integral part of any study as it sums up important arguments and its evidence to support and proves the thesis statement. Importance
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Gas Chromatography (GC) Definition: Gas chromatography is a physical method of separation in which the components to be separated are distributed between two phases‚ one being a stationary bed of large surface area‚ and the other a gas that percolates through the stationary bed. When the stationary phase is a solid‚ the separation process is more precisely called gas-solid chromatography. Introduction: Gas Chromatography is a technique widely used to separate complex mixtures of substances. Compounds
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exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography‚ in
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3.7 Nuclear Independent Chemical Shift Analysis The Nuclear Independent Chemical Shift is measured as magnetic shielding of a molecule have been calculated at the B3LYP/6-31G theory level. Aromaticities are represented by negative NICS Values and antiaromaticity is represented by positive NICS Values. The large negative NICS Values of the title compound represent stronger aromaticity [29-35].Our present investigation of the density functional calculations of doped fullerene with glycine shows several
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Objective: The goal of the experiment was to use UV/VIS spectroscopy to collect data to quantify acetaminophen‚ aspirin‚ and caffeine from a migraine tablet where there is heavy spectral interference using Target Factor Analysis1. The migraine tablet has other ingredients in it like FD&C blue #1‚ stearic acid‚ and saccharin. Saccharin is the ingredient thought to have the biggest interference and was used as the experimental basis2. Instruments: UV/VIS spectroscopy was used to analyze the absorption
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(1) Answer to Question in lab manual. NO introduction/background etc. 1. What is the retention time for caffeine? Retention time for caffeine : 4.149+4.142+4.152+4.1454 = 4.15 2. What is the retention time for benzoic acid? Retention time for caffeine : 2.651+2.817+2.857+2.9274 = 2.81 3. How are the retention times for caffeine and benzoic acid related to their chemical structure and the nature of the chromatographic
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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E-BOOKS VS PAPER BOOKS Reading has become one of the important elements in our life. We have been exposed to the books and learn how to read since we were still children. Couples of books innovations have been created since the past couple years. It started out with paper books or what we can call it as ‘traditional books’ and as the wave of publishing changes‚ we have the ‘modern books’ or known as virtual books that we can just keep it in our pocket‚ portable and flexible. However‚ some prefer
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