Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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2-butanol yielded 4.6% 1-butene‚ 67.3% trans-2-butene‚ and 28.1% cis-2-butene‚ and a dehydrohalogenation of 2-bromobutane yielded 19.1% 1-butene‚ 69.9% trans-2-butene‚ and 11.0% cis-2-butene. Backround: Gas Chromatography separates organic samples much in the same way as column chromatography. The only differences are that it uses a moving gas phase and a stationary liquid phase‚ and that the temperature of the gas system can be controlled. In a gas chromatograph the sample is shot in with a syringe
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IDENTIFICATION OF UNKNOWN SOLUTIONS USING TLC CHROMATOGRAPHY Purpose: To become familiar with the principles and terminology of TLC chromatography to identify an unknown molecule based on comparisons with known laboratory standards and Rf factors. Background Thin Layer Chromatography: Chromatography is a sophisticated method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that
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1. What physical evidence did you find at the victim’s home? A box of matches‚ a space heater‚ beer bottles‚ Natalie’s wallet‚ a V-shaped burn pattern‚ burned outlet‚ a protected surface on the ground‚ crazed glass‚ and a puddle-shaped burn pattern. 2. Describe the process that you used to take a sample of the puddle-shaped burn pattern on the floor. Why did you also take samples from the portion of the floor that was not burned? First‚ I took the chisel to loosen up some of the wood chips
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Preparation of Dibenzalacetone by the Aldol condensation David o Neill Date of experiment: 14/12/2011 Apparatus Steam bath‚ ice bath‚ Buchner funnel‚ beaker‚ conical flask‚ filter paper‚ TLC apparatus‚ Melting point apparatus Materials / chemicals Benzaldehyde‚ acetone‚ ethanolic sodium hydroxide‚ ethanol Introduction The synthesis of dibenzalacetone is formed from an Aldol condensation reaction. An Aldol condensation reaction is a very effective way of forming a carbon – carbon bond reaction‚ in
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Australian Pulp and Paper Manufacturing Industry.The current strategic study represents the case of the Australian Pulp‚ paper and paperboard manufacturing Industry. The Australian pulp and paper industry produces items that are fundamental to to everyday living for example paper and paper products for personal hygiene‚ food packaging‚ paper for printing‚ newspapers‚ and magazines. The industry is also a huge producer of renewable energy and the major user of recovered paper for recycling. The reason
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Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric glucose oxidase assay will be used to monitor the presence of glucose and a colorimetric iodine assay will be used to monitor the presence of starch in prepared solutions
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prevalence of the green chlorophyll. Anothocyanin(red or purple)‚ carotene(orange)‚ and xanthrophyll(yellow) are found in different proportions in different leaves. Leaf pigments can be separated by using paper chromatography. Paper chromatography is a technique that extracts pigments into a paper filter called chromatogram. What are the pigments are in Red Wondering Jews? Carotenoids These pigments primarily absorb in the blue wavelengths‚ allowing the longer wavelengths to be scattered and producing
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AND PARACETAMOLPRACTICAL REPORT ON THE ISOLATION AND IDENTIFICATION OF CODEINE AND PARACETAMOL AIM: To extract codeine and paracetamol from its tablet by solvent extraction and tentatively identify in comparison to standards using Thin Layer Chromatography. INTRODUCTION: Codeine or methyl morphine‚ an alkaloid‚ was first isolated in 1832 from raw opium. It concentration ranges from 0.2% to 0.8%. Mostly used for its analgesic‚ anti-tussive and anti-diarrheal capabilities (Tremlett‚ Anderson
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that might need this purification setup. Experimental Procedure: This lab follows the experimental procedure of the book as outline in the Mayo book on pages 133 and 134. However; the following modification were made to the procedure: Use gas chromatography instead of refractive index Collect GC for only fraction 1 and fraction 3 because of time constraints. Reaction Scheme: Data and Results: Compound Amount (mL) Boiling point (°C) Hexanes 1.5 62-69 Toulene 1.5 110-111
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