Separations: Chromatography of M&M and Ink Dyes Almost all substances we come into contact with on a daily basis are impure; that is‚ they are mixtures. Similarly‚ compounds synthesized in the chemical laboratory are rarely produced pure. As a result‚ a major focus of research in chemistry is designing methods of separating and identifying components of mixtures. Many separation methods rely on physical differences between the components of a mixture. For example‚ filtration takes advantage of substances
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Thin Layer Chromatography of the Unknown Analgesic Jessica Bajao*‚ Phoebe Abalos‚ Kevin Antiga‚ Carmelus Aseneta 3-Biology 2 College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The group used five different analgesics in this experiment: aspirin‚ acetaminophen‚ ibuprofen‚ caffeine‚ and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate‚ it was placed under ultraviolet light for the spots resulted
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Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
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Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
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ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3‚ 2011 Date of submission : February 10‚ 2011 FENNELL CAMPUS HAMILTON‚ ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the components of an unknown solution. The
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How Does Chromatography Work? Chromatography is a surface effect. It works by first starting off with an unidentified liquid being injected into a column. As the chemicals in the liquid move down the column‚ they separate depending on their physical and chemical attributes‚ such as speed and weight. To easily picture the process‚ the example will pertain to a paper chromatography process in which the liquid is moving over the paper‚ separating the mixture as it travels up the piece of paper. When
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tested for by a possible employer include Cocaine (crack)‚ Amphetamines (crystal)‚ Opiates (codeine‚ morphine‚ heroin)‚ PCP (phencyclidine)‚ and Marijuana. Gas chromatography/mass spectrometry is used to test hair and urine samples of possible drug abusers or job applicants‚ and it is the best method for the testing of drug use. Gas chromatography and mass spectrometry are two different methods for identifying chemical substances‚ and the two instruments have be coupled together to perform a highly complementary
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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must you use lead pencil‚ instead of a pen‚ to mark your chromatography paper? A lead pencil should be used instead of a pen because the ink of the pen would dissolve in the eluting solution and separate. The components of the ink could mix with the other ink/ dyes that would be tested on the same chromatogram. Thus‚ a lead pencil should be used. 2. Why should you avoid touching the surface of the paper to be used for amino-acid chromatography? We should avoid touching the surface of the chromatogram
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CHROMATOGRAPHY _TOPIC_ : 7.2 _RESEARCH QUESTION:_ What is chromatography? How many pigments are there in a plant? How the different pigments in a plant can be separated? _APPARATUS_ : Please refer to the handout _MATERIAL_ : Please refer to the handout _METHOD_ : Please refer to the handout _DATA COLLECTION_: Coloured band Pigment front distance (± 0.05cm) Solvent front distance (± 0.05cm) Green 11.7 13.0 Yellow 12.5 13.0 _DATA PROCESSING_: Coloured band Pigment
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