Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
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Biol 1406‚ Instructor: Alice Zhou Updated 4/18/12 Chapter 10: Photosynthesis 1. Describe the energy transformation that occurs in photosynthesis. Solar energy to chemical energy specifically stored in sugar. SolarChemical energy (sugar) 2. Write the summary equation of photosynthesis. Solar + 6CO2 +6H2O C6H12O6+6O2 (simple) Complex 3. Photosynthesis produces organic sugar molecules. Where does the carbon come from in making the sugars? From carbon dioxide 4. Aerobic
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PHOTOSYNTHESIS & CELLUAR RESPIRATION LAB REPORT % Absorption 400 425 450 475 500 525 550 575 600 625 650 675 700 Average 47 44 37 27 14 10 8 9 9 10 15 17 14 Use the graph of Average % Absorption for Grass Pigments to answer the following questions. ANSWER ONLY THE QUESTIONS BELOW. YOU WILL LOSE POINTS IF YOUR ANSWER INCLUDES UNRELATED INFORMATION. 1. What specific range of wavelengths explains why grass is green? The absorption of all wavelengths outside approximately
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Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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purpose of this lab was to investigate whether veins affect photosynthesis rate. It was predicted that having veins would decrease the rate because chloroplast might be less frequent due to the vascular tissue. For this experiment‚ 4 groups were set up: veins and no veins in CO2 solution‚ and veins and no veins in distilled water‚ the distilled water groups served as controls. These 4 groups were placed under a light bulb in order for photosynthesis to occur. No disks experience movement in the first 7
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What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
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Both photosynthesis and cellular respiration are the main pathways of energy transportation in organisms. However‚ the reactants and the products are exact opposites in photosynthesis and in cellular respiration. In photosynthesis‚ cells take in carbon dioxide (CO2) and water (H2O) by absorbing energy from the sun‚ and then the cells release oxygen (O2) and store glucose (C6H12O6). The formula of photosynthesis is: Light energy 6CO2+6H2O → C6H12O6+6O2
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possible to identify which peak correlates with which compound. Figure 2 contains a peak at around 500 and 700 nm which is a good indicator that that would-be chlorophyll b. This makes sense because this was the bottom band from the thin layer chromatography and chlorophyll b is the most polar compound. Chlorophyll B contains an aldehyde where chlorophyll A has a methyl group making it slightly more polar. Following that‚ figure 4 contains
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Misael Acosta Photosynthesis Lab Report Ms. Rocha AP Biology 4th Period II. Intro/Background 1. In order for oxygen to be present photosynthesis must occur. Photosynthesis takes in light and CO2‚ and through a series of steps produces oxygen. Photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. Examples include the production of O2 or the consumption of CO2. All the oxygen within the leaf disks will be removed before being placed
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Lab Report #1 Photosynthesis Lab Report: The Effects of Varying Concentrations of CO2 on the rate of Photosynthesis in Spinach Leaves Introduction Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct. The purpose of this experiment is to see how different concentrations of carbon
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