Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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tested for by a possible employer include Cocaine (crack)‚ Amphetamines (crystal)‚ Opiates (codeine‚ morphine‚ heroin)‚ PCP (phencyclidine)‚ and Marijuana. Gas chromatography/mass spectrometry is used to test hair and urine samples of possible drug abusers or job applicants‚ and it is the best method for the testing of drug use. Gas chromatography and mass spectrometry are two different methods for identifying chemical substances‚ and the two instruments have be coupled together to perform a highly complementary
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Separations: Chromatography of M&M and Ink Dyes Almost all substances we come into contact with on a daily basis are impure; that is‚ they are mixtures. Similarly‚ compounds synthesized in the chemical laboratory are rarely produced pure. As a result‚ a major focus of research in chemistry is designing methods of separating and identifying components of mixtures. Many separation methods rely on physical differences between the components of a mixture. For example‚ filtration takes advantage of substances
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Transporting Nutrients Pigments and photosynthesis 1. Distinguish between the strategies used by autotrophs and heterotrophs to obtain free energy for cellular processes. In other words‚ how and in what form does each capture and store that energy? 2. Explain how photosynthesis differs in eukaryotic organisms (with organelles such as chloroplasts) and in prokaryotic organisms (single-celled‚ no organelles). 3. How does a metabolic pathway such as photosynthesis suggest common ancestry? 4
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Investigation into the photosynthetic activity of isolated chloroplasts from spinach beet to various light conditions. Introduction Chlorophyll‚ an important pigment in chloroplasts‚ is an essential component in photosynthesis; converting electromagnetic radiation from the sun into potential chemical energy. Photons excite electrons in chlorophyll which move through the electron transport chain‚ resulting in energy being stored in the bonds of ATP and NADPH. The flow of electron through the electron
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In this experiment‚ the class examined how the rate of photosynthesis was affected by different light colors at different distances in shoots of a Cabomba plant. The plants were placed in two different beakers‚ one with recently boiled and cooled distilled water and the other with saturated sodium bicarbonate solution. The three different color photoflood lamps used for this experiment were white‚ red‚ and green at four distances of thirty‚ sixty‚ ninety‚ and one hundred-twenty centimeters away
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Introduction Photosynthesis takes place in the chloroplast and is the use of sunlight energy for cell processes. It happens through a number of chemical reactions and the transfer of electrons. It is used as the source of energy (ATP) for plants. The reaction equation for photosynthesis is H2O + CO2 + (Light) (CH2O)N + CO2 In the Hill reaction‚ Robert Hill showed how chloroplasts in water can still function if there is light and an electron acceptor to release oxygen. He proved that the oxygen
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light on the rate of photosynthesis Introduction This experiment was performed to investigate the effects of different wavelengths of light on the rate of photosynthesis. If a multiple colored film petri dishes are place under a 50 watt lamp‚ then the rate of photosynthesis will be greater for those with red‚ blue and clear film‚ than those with green and black film. We believed the petri dishes with the red‚ blue and clear film would produce the best results of photosynthesis as compared to the
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The lamp experiment is primarily based off of the process of photosynthesis. The experiment tracks the rate of photosynthesis as it is affected by the color of the light source. Changing the light bulb color changes the length of the wavelengths that hit the chloroplasts in the plant leaves and effects how fast or if photosynthesis occurs. The equation for photosynthesis is 6 CO2 + 6H2O + light energy=C6H12O6 + 6O2 meaning that when a plant cell absorbs carbon dioxide and water alongside sunlight
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This archive file contains BIO 240 Week 1 Photosynthesis and Respiration Paper Biology - General Biology Write a 700- to 1‚050-word paper summarizing the events of cellular respiration and photosynthesis and examine the relationship between the two. Include the following in your paper: · For photosynthesis‚ include a summary of the events in: o The Light Dependent Reaction o The Calvin Cycle (Light Independent Reaction) · For cellular respiration‚ include a
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