Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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investigate the effect of carbon dioxide concentration on the rate of photosynthesis Introduction Photosynthesis is a process that is essential for every living organism. Organic substances‚ such as glucose‚ are made from carbon dioxide and water by light energy from the sun. The light energy is absorbed by chlorophyll and converted to chemical energy. During the process‚ oxygen is released as a by-product. The rate of photosynthesis is affected by a few of factors‚ including light intensity‚ temperature
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What Factors Affect the Rate of Photosynthesis in living leaves? Abstract: In this lab‚ four different types of leaves were tested to see the rate at which each leaf photosynthesized. This lab demonstrates how plants store light‚ capture light‚ and use light as energy for reproduction and growth‚ by photosynthesis. The control in this experiment was spinach‚ which was tested before any of the other plants were. The other plants that were tested were English Ivy‚ C4 Plant‚ and a multicolored
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This experiment focuses on the effect of light intensity on the rate of photosynthesis. In simple terms this refers to how the production rate of the products of photosynthesis (oxygen and glucose) will be affected by a light source at different distances. The hypothesis states that “If there is a higher light intensity‚ then more oxygen and glucose molecules will be produced‚ and the rate of photosynthesis will increase.” This hypothesis was accepted. This was determined by counting the number of
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Discussion Using the Thin Layer Chromatography experiment‚ caffeine was found to be the most polar due to the fact that it stayed closer to the stationary phase. Caffeine contains four amine groups that are extremely polar as a result of the hydrogen bond and amide functional group. Acetaminophen was found to be the second most polar analgesic drug tested. Acetaminophen contains a polar alcohol group on one side and amide group on the other but also includes non-polar functional groups that consisted
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The correlation between the occurrence of photosynthesis and different lengths of light waves. Introduction Photosynthesis is an exceptionally interesting process that is used by some organisms and plants. In the conversion of light energy from the sun to chemical energy that is used as food‚ photosynthesis is used for the everyday undertakings by some organisms and plants during their life span. This complex method of energy production is also represented by the equation: 6CO2 + 6H2O C6H12O6
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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relationship of different wavelengths of light and the rate of photosynthesis in spinach leafs. The rate of photosynthesis was measured every five min under light colors of white‚ green‚ red‚ blue and yellow under a light intensity of 2000 lux. The rate of photosynthesis was measured by the spinach disk method in which we replaced the air from the disks with sodium bicarbonate using a vacuum. Under photosynthesis‚ oxygen‚ a product of photosynthesis‚ replaced the bicarbonate solution‚ made the disks less
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Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it
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