Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe
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Introduction Photosynthesis is the process by which autotrophic organisms convert light energy to chemical energy in the form of glucose. Without this process life on earth would be very different because every organism directly or indirectly depends on photosynthesis. Photosynthesis helps the plants to grow roots‚ leaves‚ flowers and fruits. It is an extremely important process because plant tissue serves as the bottom of every food chain. The process of photosynthesis is dictated by a number
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References: 1) E. Stahl‚ Thin Layer Chromatography‚ Springer-Verlag‚ New York‚ 1969 2) Camag Scientific‚ Inc.‚ 515 Cornelius Harnett Drive‚ Wilmington‚ NC 28401‚ phone 800-3343909‚ fax 919-343-1834 3) Desaga GmbH‚ P.O. Box 101969‚ D-69009 Heidelberg‚ Germany‚ phone 0-62-21-83590‚ fax 0-62-21-840887
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Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter
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Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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Separating Water Soluble and Acetone Soluble Inks through Chromatography Abstract: Chromatography is a term used to define a set of laboratory techniques for the separation of mixtures. Through Chromatography we analyzed complex mixtures. In our recent lab we analyzed complex mixtures. The purpose of this lab was to find whether the water soluble or acetone soluble liquid traveled farther. In our recent lab we measured the distance traveled by the different colored water and acetone soluble
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electron transport chain because each carrier in the chain has a greater tendency to capture and hold electron than the carrier before it. PHOTOSYNTHESIS Photosynthesis which requires the energy of sunlight by the green chlorophyll pigment for the manufacture of carbonhydrate‚ carbondioxide and H2O. Van Neil propose a general equation for photosynthesis as follows: CO2 + 2H2A light (CH2O) + H2O + 2A Carbon Hydrogen carbonhydrate Water Dioxide donator
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project? Topics: Photosynthesis (chapter 7) and Cellular Respiration (chapter 8) Objectives: to explain and describe the metabolism of photosynthesis reactions and respiration reactions Specific objectives: (each one is a topic project) to explain the overall reactions of photosynthesis and respiration as metabolic pathways and coupled reaction. to explain and describe photosynthesis light dependent reaction and photosystems I and II in the thylacoid to explain and describe photosynthesis light independent
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