Drugs screen using Thin Layer Chromatography of basic illicit drugs Abstract The majority of evidence submitted to crime labs comes from drug-related crimes. Often‚ this evidence includes unidentified powders that may be illegal drugs. In order to prosecute individuals for possession of illegal substances‚ it is necessary for forensic scientists to positively identify any suspected drugs submitted to the laboratory. In addition‚ forensic toxicologists must determine the identity
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Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined
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must you use lead pencil‚ instead of a pen‚ to mark your chromatography paper? A lead pencil should be used instead of a pen because the ink of the pen would dissolve in the eluting solution and separate. The components of the ink could mix with the other ink/ dyes that would be tested on the same chromatogram. Thus‚ a lead pencil should be used. 2. Why should you avoid touching the surface of the paper to be used for amino-acid chromatography? We should avoid touching the surface of the chromatogram
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Extraction Important Peaks Concluded Compound C-5 #1 -CH‚ C=O cyclopentanone C-5 #2 -OH‚ -CH 1-hexanol D-4 #1‚2 C=O‚ -CH Cannot conclude Preparative Gas Chromatography was used to separate volatile compounds‚ which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components
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as the graphical representation of the rate of photosynthesis against wavelengths of light. The graph shows how effective the different wavelengths of light are at enabling photosynthesis‚ and this can be determined by measuring either the carbon dioxide consumed or by the amount of oxygen released. Chlorophyll is a pigment found in the chloroplasts of organisms. In plants‚ it is the chlorophyll that absorbs the light energy and drives photosynthesis. On the absorption spectrum‚ chlorophyll a and
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Title: The success of photosynthesis in geranium leaves using visible light wavelengths obstructed by black paper‚ and red‚ blue‚ and green translucent filters. I. Abstract Photosynthesis is a process in which plants use light‚ water‚ and carbon dioxide to produce sugars‚ water and oxygen. Chlorophyll a and chlorophyll b‚ in the chloroplasts of a leaf‚ are responsible for absorbing wavelengths of light for use in photosynthesis with red and blue wavelengths being optimal. Testing
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Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
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Title:The Chromatography Process of Expo Markers Abstract:This experiment is to uncover whether or not the Expo Markers are homogeneous or heterogeneous mixtures. The experiment will conduct filter paper being slightly submerged in a container of water and inspected for pigmentation changes. In the experiment the pigments will rise up the filter paper once exposed to the water (solvent). After the experiment‚ it concludes that the mixture is a homogeneous mixture due to the chemical
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Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted with the
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