experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to observe the effect of the increasing polar eluent fractions‚ to analyze the different fractions collected‚ and to correlate the structure of the components with polarity and behavior on a chromatographic column. Background The best method for separating mixtures in organic chemistry is chromatography. Chromatography is the separated of a mixture of two or more
Premium Chromatography Solubility Solvent
one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the data was graphed it clearly showed the higher rate of red light absorption over green light. These results along with previous research indicate the importance of red light in photosynthesis and the minor role green light plays. The majority of life on Earth depends on photosynthesis for food and oxygen. Photosynthesis is the conversion of carbon
Premium Photosynthesis Electromagnetic radiation Chromatography
They use the absorption of light to aid in photosynthesis‚ and protect chlorophyll from damage. In humans‚ Lycopene acts as an antioxidant. Lycopene is found in various fruits and vegetables. The purpose of this experiment is the isolation of Lycopene from tomato paste. This is done using liquid/solid extraction and chromatography. Once the Lycopene is isolated‚ IR spectroscopy will determine its percentage actually obtained by chromatography. Procedure: A massed sample of 1.012g of
Premium Lycopene Laboratory glassware Photosynthesis
The Effect of Light Intensity on Photosynthesis and Transpiration Inna V. Raymundo BOTANY I Section V-6L August 29‚ 2012 ¹A scientific paper submitted in partial fulfillment of the requirements in Botany I laboratory under Dr. Nonnie Bautista‚ 1st sem‚ 2012-2013. ABSTRACT Photosynthesis and transpiration both play important roles in plants. But there are factors which affect the rate of photosynthesis and transpiration. The external factor that was identified is the
Premium Plant Photosynthesis Pigment
The Effect of Light Intensity The effect of different light wavelengths was tested in the following experiment. The rate of photosynthesis can be affected depending on the light treatment it receives. With the aid of DPIP acting as NADPH in photosynthesis‚ it was possible to observe the reaction as it took place. Samples were created with dark‚ 24 cm‚ 30 cm‚ and 49 cm light treatments. The absorbance was measured in five-minute intervals for each sample and recorded in an appropriate table
Premium Light Wavelength Chlorophyll
Lab 11- Plant pigment and Photosynthesis Zelda Salfati December 20th‚ 2012 BIOL 1012Y Partners Lab: Nicole Mintz‚ Bayla Kronman
Premium Photosynthesis
BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
Premium Photosynthesis Metabolism Adenosine triphosphate
2013 Group No.: 8 Date Submitted: January 6‚ 2014 Experiment 4: Extraction and Chromatic Separation of Plant Pigments from Tomato Paste ABSTRACT In column chromatography a mixture is dissolved in a solvent and poured over a column of solid adsorbent then eluted with the same or a different solvent. This method is often used for preparative purposes; when a relatively large amount of the mixture and the components
Free Chromatography
Jamie Milano Biology 150 Photosynthesis Homework April 8‚ 2013 Reviewing Your Knowledge 2. A pigment solution contains compound A with 4 polar groups and compound B with 2 polar groups. You plan to separate these compounds using paper chromatography with a non-polar solvent. Predict the location of the two bands relative to the solvent front. Explain your answer. Since compound A is probably more polar than compound B‚ compound B will move further in the chromatography. Since the solvent is non-polar
Premium Chlorophyll Photosynthesis Visible spectrum
Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
Premium Photosynthesis Color Green