Photosynthesis On-line Lab PART A. Separation of Plant pigments Access the following website: http://www.phschool.com/science/biology_place/labbench/lab4/intro.html (you will need to "cut and paste" this into your browser) You should see the Plant Pigments & Photosynthesis lab. Under the figure of the notebook‚ click the "next" button. Read "Key Concepts I: Plant Chromatography" Then click "next" and read "Design of Experiment I" Then click "a closer look". Read "Depositing the
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absorb it. 2. List two variables besides the wavelength (color) of light which might affect the rate of food production in plants. Light intensity and carbon dioxide 3. Why is chlorophyll important for all biological life? It is necessary for photosynthesis which creates food for plants which is food for animals. 4. A. In Part I of the procedure‚ what is the name of the indicator used to identify the presence of CO2? Phenol Red 4. B. What color did the indicator turn after blowing air into the
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best. The idea is to be able to isolate the protien that is in interest from the protiens that are not needed. Below are several chromatography techniques that are used to analyze proteins. Size exclusion chromatography (SEC) In this type of technique the protein is separated according to the size of the protein. The matrixes used in size exclusion chromatography have a range of beads with different pore sizes (H. Dai). The seperation of the protein is dependent on how the protein can enter
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Maldonado Abstract: In this lab the students did two labs in which they learned the process of photosynthesis‚ which involves the use of light energy to convert carbon dioxide and water into sugar‚ oxygen‚ and other organic compounds. Oxygen is released as a product. This process is often summarized by the following reaction: 6CO2 + 12H2O ------> C6H12O6 + 6H2O + 6O2 The energy of photosynthesis comes from absorbed photons found in light and involves a reducing agent‚ in this case water. The
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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Separations ........ 413 16.2.5 Multidimensional Planar Chromatography (Unidimensional Multiple Development and Two-Dimensional Development)..... 415 16.2.6 Quantitative Evaluation................................................................ 418 16.3 Modern TLC Techniques in the Separation of Flavonoids ....................... 418 16.3.1 Overpressured-Layer Chromatography ........................................ 418 16.3.2 Rotation Planar Chromatography................................................
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of the GFP protein. This indicates that the molecule responsible for the observed green trait was protein. The transformation efficiency was found to be 793.75 transformants per microgram. The protein was filtered out of the cell and column chromatography was used as a means to isolate the protein by taking advantage of its hydrophobic properties. This was performed by washing the column containing the GFP protein amongst other proteins with buffers varying in salt concentrations. As expected
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THE STUDY OF CHLOROPHYLL CONTENT IN VARIOUS PLANTS A Project Report Submitted by SANDEEP SETHI In partial fulfillment of the CBSE GRADE XII IN BIOLOGY AT 2015-16 CERTIFICATE This is to certify that Sandeep Sethi of grade XII‚ ST. XAVIER INTERNATIONAL SCHOOL‚ BHUBANESWAR with Registeration Number _______________________ has satisfactorily completed the project in Biology on STUDY OF CHLOROPHYLL CONTENT IN FIVE DIFFERENT SPECIES OF PLANTS‚ in partial fulfillment of the requirements
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Fluorenone (2) was separated by column chromatography. Chemical Equation: Fluorene (1) Fluorenone (2) Mp 114C mp 83C MW 166.22 MW 180.21 Introduction: Column chromatography was invented by Tswett in 1906 so that his study of the chemistry of chlorophyll could be facilitated. Tswett made significant advances in the method and demonstrated its applicability to the separation of many different kinds of mixtures. Column chromatography rapidly found application in all areas
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E5 & E6 Extraction of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach.
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