of the GFP protein. This indicates that the molecule responsible for the observed green trait was protein. The transformation efficiency was found to be 793.75 transformants per microgram. The protein was filtered out of the cell and column chromatography was used as a means to isolate the protein by taking advantage of its hydrophobic properties. This was performed by washing the column containing the GFP protein amongst other proteins with buffers varying in salt concentrations. As expected
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antioxidant. Lycopene is found in various fruits and vegetables. The purpose of this experiment is the isolation of Lycopene from tomato paste. This is done using liquid/solid extraction and chromatography. Once the Lycopene is isolated‚ IR spectroscopy will determine its percentage actually obtained by chromatography. Procedure: A massed sample of 1.012g of tomato paste was placed in a 125mL Erlenmeyer flask. To the flask‚ 5mL of 50:50 hexane-acetone was added into the flask. After the 50:50
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An Example of Using Key Performance Indicators for Software Development Process Efficiency Evaluation. Abstract - This paper gives an overview of possible Key Performance Indicators (KPI) that can be used for software process efficiency evaluation. The overview is based on currently used KPIs in software development projects on CPP platform. The most important KPIs are analyzed‚ and their usage in the process efficiency evaluation is discussed. The outcome of the measurement is used to
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Fluorenone (2) was separated by column chromatography. Chemical Equation: Fluorene (1) Fluorenone (2) Mp 114C mp 83C MW 166.22 MW 180.21 Introduction: Column chromatography was invented by Tswett in 1906 so that his study of the chemistry of chlorophyll could be facilitated. Tswett made significant advances in the method and demonstrated its applicability to the separation of many different kinds of mixtures. Column chromatography rapidly found application in all areas
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Info from case total revenue for last reporting = 110 million cio reviewed 3 following implementation strategies: -classic disintermediation - removal of intermediaries in a supply chain. connects supplier directly with customers -remediation-working more closely with ecisting middlemen partners. strategy could be affected by high contracting risks. -network-building alliances and partnerships with both existing and new suppliers and distributors involving a complex set of relationships
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advantages and disadvantages of Agile Software Engineering? One mooted solution to project management issues is Agile Software Engineering. Agile software engineering was devised‚ in the early 1990’s‚ as a counter to the then-prevalent preference for huge‚ carefully-planned and expertly orchestrated grand projects. As Alistair Cockburn has noted‚ "the 1990’s saw some of the most bloated‚ over-organised attempts at problem-solving imaginable‚ whereas agile software engineering came in like a breath of
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Appearance of solution. Dissolve 1.0 g in 9 ml of alcohol R. The solution is clear (2.2.1) and colourless (2.2.2‚ Method II). Related substances. Examine by liquid chromatography (2.2.29). Prepare the solutions immediately before use. Test solution. Dissolve 0.10 g of the substance to be examined in acetonitrile for chromatography R and dilute to 10.0 ml with the same solvent. C. N‚N′-diacetyl-L-cystine‚ Reference solution (a). Dissolve 50.0 mg of salicylic acid R in the mobile phase and dilute
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What is Software Defined Radio? With the exponential growth in the ways and means by which people need to communicate - data communications‚ voice communications‚ video communications‚ broadcast messaging‚ command and control communications‚ emergency response communications‚ etc. - modifying radio devices easily and cost-effectively has become business critical. Software defined radio (SDR) technology brings the flexibility‚ cost efficiency and power to drive communications forward‚ with wide-reaching
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International Journal of Business and Management Vol. 6‚ No. 5; May 2011 Jordan Software Industry: Investigating the Role of Human Capital Ashraf Bany Mohammed Faculty of Information Technology‚ Middle East University P.O. Box: 383‚ Amman 11831‚ Jordan Tel: 962-777-588-997 Received: November 19‚ 2010 E-mail: abany@meu.edu.jo Accepted: December 22‚ 2010 doi:10.5539/ijbm.v6n5p217 Abstract Software industry is realized as one key opportunity for socio-economic development‚ especially
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Bhindia Pooja Rayel Title : Paper Chromatography Photochemical Smog Lecturer’s Name : Ms. Usha Nanthini Table of Content No. | Content | page | 1. | Paper Chromatography | | | 1.1 Definition | | | 1.2 Background | | | 1.3 Rf Values | | | 1.4 Principles of Paper Chromatography | | | 1.5 Procedure of Paper Chromatography | | | 1.6 Uses of Paper Chromatography | | | 1.7 Application of Paper Chromatography | | 2. | Photochemical Smog | | | 2.1
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