experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to observe the effect of the increasing polar eluent fractions‚ to analyze the different fractions collected‚ and to correlate the structure of the components with polarity and behavior on a chromatographic column. Background The best method for separating mixtures in organic chemistry is chromatography. Chromatography is the separated of a mixture of two or more
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Candy Chromatography Background info: Paper chromatography is a logical technique used to separate works of a solution. Three examples of how we apply this technique to real-life would be: contaneminants in water‚ separation of plant pigmentation‚ and analysis of narcotics. Source: http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html#b Purpose: To find out why candies are different colors. * Materials: Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
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To purify the protein in the cell lysate from lab 1 through nickel affinity chromatography. Protein purification should result in only one type of protein ideally‚ which is the protein of interest‚ wt-DHFR and mut-DHFR in this case. A pure protein allows for further analysis on the protein to be conducted‚ such as its concentration (Bradford assay)‚ its molecular weight‚ and its biological activity. 2. Overview of experiments Buffer Preparation Add the liquid sodium phosphate‚ solid sodium chloride
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procedures of this laboratory will be used to gain a conceptual and practical understanding of separation techniques used to isolate monomers from their respective polymers. Namely‚ the techniques that will be explored are dialysis and gel filtration chromatography. In the remaining two experimental procedures‚ colorimetric assays will be used to detect the presence of certain carbohydrates. Glucose oxidase and Iodine Reactions will be performed in conjunction to demonstrate the procedure of such assays
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Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
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Title: Competing Nucleophiles (Exp 24‚ pp 211-221‚ pp 808-823‚ pp 836-842) Purpose: The purpose of this experiment is to determine the nucleophilic strength of chloride and bromide ions as it reacts with 1-butanol (n-butyl) and 2-methyl-2-propanol (t-butyl alcohol) under SN1 and SN2 conditions. Method: 40 g of ice and approximately 30 ml of sulfuric acid is cautiously added to a 100 mL beaker respectively. Weigh 7.6 g of ammonium chloride and 14.0 g of ammonium bromide and place it in
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different chemical properties of each compound. We will accomplish this by a separation procedure known as distillation‚ which relies on each compound having a distinct and separate boiling point. Our pure products will be analyzed with gas chromatography to determine the success of the distillation. Procedures The experiment was performed as stated in the course textbook: Pavia‚ D. L.‚ Lampman‚ G. M.‚ Kriz‚ G. S.‚ Engel‚ R. G. Introduction to Organic Laboratory Techniques: A Microscale Approach
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Software Reliability Engineering: A Roadmap Michael R. Lyu Michael R. Lyu received the Ph.D. in computer science from University of California‚ Los Angeles in 1988. He is a Professor in the Computer Science and Engineering Department of the Chinese University of Hong Kong. He worked at the Jet Propulsion Laboratory‚ Bellcore‚ and Bell Labs; and taught at the University of Iowa. He has participated in more than 30 industrial projects‚ published over 250 papers‚ and helped to develop many commercial
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experience the release of excess gases within a thirty-minute time period. The white light experienced the most success by concluding the thirty minutes with eight floating disks out of ten. The Activity of Photosynthetic Pigments Silica gel chromatography can be used to determine what light wavelengths are used for photosynthesis. In this experiment‚ chlorophyll a‚ chlorophyll b‚ carotene‚ and xanthophyll were tested. The distance moved by molecule and the distance moved by the solvent is used
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raise by less than 0.06% as he is larger than an average male. 6. Name three methods for drug screening describe the advantages and disadvantages of each. Name Advantages Disadvantages Immunoassays High sensitivity Not 100% specific Thin Layer Chromatography Can identify hundreds of compounds in one run and is inexpensive Labour intensive and highly technical Ultraviolet-Visible Spectrophotometry Reliable compound recognition Cannot identify less
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