Computational Systems Biology Computational Systems Biology Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations
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1. A patient with poorly controlled Type I diabetes has blood drawn and finds that the pH of his blood is 7.1. The normal pH of blood is 7.4. The patient’s blood [H+] is A) one fourth normal B) one third normal C) half normal D) twice normal E) three times normal 2. The Henderson-Hasselbalch equation: A) allows the graphic determination of the molecular weight of a weak acid from its pH alone. B) does not explain the behavior of di- or tri-basic weak acids C) employs
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The enzymes meant to digest your food and nourish you turned on you and started eating your body instead. In your bloodstream was a virus that was very specific to Alpha-1 Antitrypsin‚ the substance that inactivates Trypsin‚ Chymotrypsin‚ and Neutrophil Elastase. I know that’s all Chinese to you but you’ll soon understand. I decided to play a game with you‚ the same way you played games with me. I decided to trick your body into thinking it was doing what it was supposed to. My
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Bio 6 (Mon. Lab) 5/6/13 The Effects of Pepsin VS. Trypsin in The Digestion of Protein Introduction As food is mechanically and chemically digested through our oral cavity then passes through our pharynx and down our esophagus‚ our food then enters the stomach. The stomach‚ being the main organ for storage‚ also helps with breaking down our food‚ but in order to accomplish that our pancreas helps by excreting an inactive digestive enzyme called pepsinogen. Pepsinogen is the inactive form
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Experiment #1: Carbohydrate Digestion • Tube 1 Digestion Lab – 3 ml water • Tube 2 – 3 ml 0.2% amylase • Tube 3 – 3 ml 0.2% amylase + 10 drops of 1.0M HCl • Tube 4 1 2 4 3 – 3 ml 0.2% amylase – place in hot water bath for 5 min Experiment #1: Carbohydrate Digestion • Add 5.0 ml starch solution to each tube • Incubate in 37°C bath for 1.5 hr • Divide contents of each tube evenly into 2 tubes – Lugol’s Test – Benedict’s Test Experiment #1: Carbohydrate
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EXPERIMENT 13 DIGESTION IN THE SMALL INTESTINES ------------------------------------------------- ABSTRACT The small intestine serves as the site of major digestive and absorptive processes. In this experiment‚ the action of pancreatic enzymes on representative samples of each food group under different conditions‚ such as increased/decreased pH and presence of other substances‚ were observed. A pancreatin solution was first prepared from a hog pancreas and was completely neutralized using
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enzymes include salivary and pancreatic amylase‚ present in the mouth and small intestine‚ maltase and lactase which are also present in the small intestine and are involved with carbohydrate digestion. Pepsin which is present in the stomach and chymotrypsin present in the small intestine both involved with
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The digestive tract is a continuous tract throughout the body that interfaces with the environment at both its origin ‚the oral cavities‚ as well as‚ at its termination‚ the anus. It is divided into different segments beginning with the oral cavity and followed by the pharynx‚ esophagus‚ stomach‚ small and large intestine‚ and the anus. There are also accessory digestive organs involved including the salivary glands‚ liver‚ pancreas‚ and gallbladder. Digestion begins in the oral cavity where
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Energy in context Introduction Digestion is the breakdown of large insoluble food molecules into smaller molecules so they can pass though the intestinal wall into the bloodstream and be transported throughout the body. There are seven different food groups in a balanced diet which should include Carbohydrate‚ fat‚ water‚ protein‚ fibre‚ vitamins and minerals. Although most foods contain these in some shape or form the foods that contain most of one type fall into that category‚ a chicken
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DIGESTION OF CARBOHYDRATES Digestion of carbohydrate begins in the mouth‚ with the secretion of the enzyme salivary amylase from the serous cells of the salivary gland. This enzyme breaks starch and glycogen into disaccharides. The mucous cells of the salivary gland secrete a mucus‚ which causes the food to stick together‚ and acts as a lubricant to aid in swallowing. The salivary glands are grouped into three categories: the parotid gland‚ submandibular glands‚ and sublingual‚ all located
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