Most commonly‚ Citrobacter freundii lives as a normal occupant in the feces from the intestinal tract of humans and animals (1). C. freundii can also be found in soils‚ water‚ sewage‚ and food (1). Being a facultative anaerobe‚ C. freundii can live in both the absence and presence of oxygen (1). The bacterium’s optimal temperature is 37ᵒC (1). C. frundeii is also a chemoorganotrophic‚ which means that the microorganism requires an organic source of carbon to oxidize chemical bonds from for its
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31 October 2011 Identification and observations of Citrobacter Freundii Introduction Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections‚ diarrhea‚ and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly‚ it is
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on
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Introduction The science of microbiology is all about microorganisms‚ what they do and how they work. Microorganisms are ubiquitous‚ and are a very important part of life everywhere since they have been around for billions of years. The two main themes associated with microbiology are (1) having an understanding of the nature and function of the microbial world‚ and (2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab
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References: Citrobacter amalonaticus A cousin to Citrobacter Freundii. http://www.citrobacterfreundii.org/citrobacter-amalonaticus/citrobacter-amalonaticus/ Nye‚ Jeff. Citrobacter amalonaticus. http://web.mst.edu/~microbio/BIO221_2010/C_amalonaticus.html Virtual Unknown Software
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Name: Madison Gullatt Sect/Table: !/D Experiment 8 Report: Extracellular Enzymes Purpose: The purpose of this experiment is to perform a variety of different test and record the data from each test in relation to extracellular enzymes. Results: 1. Fill in the following tables with your observations. Note if the test is positive or negative for the enzyme. Also note the appearance of the colonies and/or media. This will include: the color of the medium immediately surrounding
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Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
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Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The
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HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports.
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Unknown #11: Citrobacter freundii Tryptic Soy Agar Test (TSA): TSA is a basic medium that is most similar to nutrient agar. The agar contains carbon‚ nitrogen‚ sodium chloride‚ and agar. This allows for the growth of a large variety of microorganisms to grow and ferment in the medium. Mannitol Salt Agar (MSA): MSA is a selective and differential medium that favors the growth of salt loving organisms. It is commonly used to distinguish the different species of Staphylococci. If an organism ferments
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