The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Neurophysiology Lab Report Anatomy & Physiology Lab Report Exercise 3 Activities 1-4‚ 8 By Laurence Blake 2/27/12 A. Objective I. Activity 1-4: Eliciting a Nerve Impulse • Investigate what kinds of stimuli stimulate action potential. II. Activity 8: Nerve Conduction Velocity • Determine and compare the conduction velocities of different types of nerves. B. Introduction I. Activity 1-4: Eliciting a Nerve Impulse • In this experiment‚ we
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Compare the Rate of Carbon Dioxide Production by Yeast under Anaerobic Conditions using different Carbohydrate Substrates. Hypothesis. The hypothesis that I draw is that "" out the five carbohydrate substrates that I will use‚ Glucose will produce the highest volume of Carbon Dioxide at every five-minute interval. Null Hypothesis. The null hypothesis that I am composing is that "" the five carbohydrate substrates that I am to use will not produce any Carbon Dioxide. Scientific Research. Under
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The lab required pressing two toothpicks against the skin simultaneously. It was difficult to press down both toothpicks at the same time and this skewed the results. When the toothpicks weren’t pressed down at the same time‚ it felt like one point‚ then another
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is unlimited per the lab manual.(Bluedoor) If there is no competition in the water‚ the growth can be unlimited. The population will have favorable
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| | Kinetics Author: Katie Wood Instructor: Donald Kavanagh Chem 106b‚ Section 001 Lab Performed 8th‚ 2012 Lab Report Submitted February 22nd‚ 2012 Abstract The purpose of the lab was to determine the order of reaction for the dye Red #40. By measuring the reaction rate between bleach and the dye‚ the order of the reaction was determined to be first order. Introduction The study of kinetics is important for studying the amount of time it takes for a particular reaction to reach
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(100 ºC) and an acidic pH. Enzyme C produced the most maltose at 37 ºC (body temperature) and a pH of 7 (neutral). Conclusion In a prior lab‚ it was given that the enzyme Aspergillus oryzae (fungus) works best at warm temperatures and an acidic pH‚ porcine (pig) works best at body temperature (37 ºC) and
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9/23/12 Lab Report #1 Meter Reading Summary The objective of this experiment was to learn how to read different meters like the D.C. volt meter and the D.C. amperes meter. In all meters each big line is a major division and each little line in between is a minor division‚ and if there is a line smaller than the minor division lines then that would be a sub minor division. Each meter has a low‚ medium‚ and high range. For example on the D.C. volt meter the ranges go from top to bottom 150‚ 15‚
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Reshmi Radhakrishnan‚ TA: Jared Tues Rm 24 Experiment 6 Purpose: To analyze if diploid yeast strain heterozygous for ARG8 ADE2 HIS3/arg8 ade2 his3 on chromosome XV and TRP1/trp1 on chromosome IV irradiated with UV light underwent mitotic recombination to form homozygous recessive ade2/ade2 mutants. The ade2/ade2 mutant are selected then plated on different media to observe if the homozygous recessive mutants were due to mitotic recombination. Controls: If all the red cells grew on the CSM-Trp
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Title: Inhibition of Yeast Glycolysis Abstract: The purpose of this experiment was to study carbon dioxide emissions from yeast‚ as well as their respiratory rate and to use that data to study how glycolysis inhibitors affect the respiratory rate. In our experiment‚ we tested how 8.75% glucose + 1.25% NaCl‚ 8.75% glucose + 1.25% glucose-6-phosphate‚ 8.75% glucose + 1.25% citric acid‚ and yeast solution‚ all mixed with distilled water‚ affect carbon dioxide volumes and respiratory rate. Our results
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