Discussion: 6. The ph solutions that were used were 2‚4‚7‚9 and 10. All of these solutions affected the beetroot‚ but the most acidic solutions were the ones that did the most damage to the membrane. This is because the ph affects the proteins within the phospho lipid bi-layer. The acidic ph breaks the hydrogen bonds therefore denaturing the proteins and allowing beetroot pigment to secrete form the cell. 7. The ethanol solutions that were used were 11%‚ 25% and 50% ethanol. The beetroot cell
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Introduction Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is: Where [A] and [B] are the concentration of the species in the reaction. The variable k is the rate constant‚ which is a function of time and catalyst presence. The variables m and n are the order of reaction for their respective species concentration. The higher the value of the reaction order the
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1.0 ABSTRACT The production of reducing sugars by acid of carbohydrate cereals powder‚ jams (total sugar content) and jams (reducing sugar content) were performed in order to study reducing sugar production. The study involved hydrolysis carbohydrate cereals powder and jams by immersing in boiling water at environmental temperature. Acid hydrolysis was carried out through reflux boiling for 20 minutes with 10ml 0f 1.5M concentrations of diluted sulphuric acid. Reducing sugars concentrations
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The effect of increasing substrate concentration on rate of an enzyme reaction. Enzymes are biological catalysts that lower a reactions activation energy making possible many of the reactions needed for life to exist. Enzymes have a high specificity which have been explained by many theories such as Fischer’s lock and key. Currently the most widely accepted theory is the induced fit hypothesis proposed by Koshland in 1958. This hypothesis solves some of the problems with the Lock and key theory
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Title Membrane Permeability Aim To determine the effect of various chemicals and high temperature on the membrane permeability of beetroot Hypothesis Organic solvent and high temperature will destroy the cell membrane and make it permeable to the red pigment. The higher the concentration of the alcohol‚ the greater will be this effect. Principle Beetroot is the material used in this experiment to demonstrate the effects of high temperature and chemicals on the permeability of the cell membranes
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20oC‚ 40oC ‚ 60oC and 80oC Each time a piece of beetroot is removed from the heated water‚ it will be left in the distilled water for exactly 30 minutes‚ before being discarded. The fluid in each of the test tubes will be analysed using a colorimeter and compared against the control‚ which is distilled water to check for any variations in the colour of the water. The variables kept constant • The same diameter corer is used so to keep the surface area of each beetroot piece the same
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An experiment to determine the amount of urea in a specimen of urine. Introduction. Metabolism produces a number of toxic by-products‚ particularly the nitrogenous wastes that result from the breakdown of proteins and nucleic acids. Amino (NH2) groups are the result of such metabolic reactions and can be toxic if ammonia (NH3) is formed from them. Ammonia tends to raise the pH of bodily fluids and interfere with membrane transport functions. To avoid this the amino groups are converted
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It affects the titrant value because the end point of the complexometric titration is determined by what I perceived as; therefore I should use a colorimeter or other computerized measurement devices instead. Lastly‚ there was a large amount of hydroxy naphthol blue indicator (HNB) being used for complexometric titration. The HNB indicator affects the volume of solution and hence the concentration in
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PRACTICAL 7 TITLE : DETERMINATION OF ERYTHROSINE CONCENTRATION USING UV- VISIBLE SPECTROPHOTOMETER INTRODUCTION In chemistry‚ spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light‚ near-ultraviolet‚ and near-infrared‚ but does not cover time-resolved spectroscopic techniques. Spectrophotometry
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To investigate the effect of temperature on an enzyme controlled reaction Introduction and planning For the investigation of enzymes‚ I am going to conduct an experiment to see how temperature can affect the rate of reaction of enzymes by testing it with starch. The enzyme that we are going to use is called amylase. We are going to test this enzyme with starch. By mixing amylase and starch solutions together under different temperature conditions‚ we can record the rate of reaction by taking a
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