amino acids Protein fragment showing peptide bond Basis of Spectrophotometer Measuring amount of substance in solution by helping of solution color is called colorimetry. Devices that used for this method is called colorimeter. In colorimetric measurements‚ color of solution is compared with color of standard solutions in different concentrations. Measuring amount of substance in solution by helping intensity of transmittance light from solution is called photometry
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procedures; calibration of equipment‚ e.g.pH meters‚ graduated pipettes; maintaining equipment‚ e.g. burettes and Bunsen burners; collection/transport of substances and equipment for disposal; use of centrifuges; instrumentation techniques‚ e.g. colorimeter‚ electrophoresis; desiccators and vacuum storage; handling and disposal of radioactive substances; handling and use of glassware; handling of solvents and poisons;
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An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin. Aim: This investigation was on the effect temperature has on the rate that the enzyme trypsin hydrolyses its substrate‚ a protein found in milk (casein). This investigation was conducted under controlled conditions‚ the temperature being the changeable variable. Trypsin and its substrate (powdered milk which is a source of the protein casein) were heated in a water bath. The contents of the two
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HS1103- Engineering chemistry - I KINGS COLLEGE OF ENGINEERING Department of Science and Humanities Sub. Code: HS1103 Sem/Year: I / I Sub. Name: Engg.Chemistry-I UNIT – I – WATER TREATMENT PROCESS: PART – A ( 2 Marks) 1. Define hardness of water. 2. Distinguish between carbonate hardness and noncarbonate hardness. 3. Draw the structure of EDTA. What happens when EDTA is added to hard water? 4. Define alkalinity. 5. Why is water softened before using in boiler? 6. What is meant
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Biology Coursework Hypothesis: To investigate the effect of different concentrations of ethanol on the permeability of beetroot cell membranes. Prediction: By exposing a membrane to a solvent‚ ethanol‚ it will increase its permeability. So the higher the concentration of the solvent‚ the more permeable the membrane will be. But if the concentration of the ethanol is beyond a certain limit‚ it may break down the cell membrane to the point where there is in fact no overall effect as the ethanol
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Microorganisms are classified according to their structure. By means of flow charts‚ diagrams and tables explain the differences between Viruses‚ Bacteria‚ Cyanobacteria‚ Achaea and Fungi. Bacteria or bacterium are unicellular microorganisms. They are essentially only a few micrometres long and form of various shapes including the spheres‚ rods and spirals. A BACTERIAL CELL Illustration courtesy of Wikipedia. A Virus (from the Latin noun virus‚ meaning toxic or poison) is a sub-microscopic
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The Effects of varying p-Nitrophenyl Phosphate concentrations on Alkaline Phosphatase Activity Nathan Overholt Louis Settembrino March 5‚ 2002 Abstract: The enzyme Alkaline Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time assay was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm‚ and the Km was found to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction:
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The reaction rate can be studied by measuring the time taken for sufficient quantity of yellow sulfur to be formed. Over time‚ a ‘’cross’’ marked on a piece of paper under the conical flask gradually disappears when viewed from above. 1 time taken for the ‘’cross’’ to disappear because the amount of sulfur formed is considered fixed. To study the order of reaction‚ the experiment is repeated by varying the concentration of each of the reactants (Na2S2O3
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Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk INTRODUCTION: Enzymes are proteins which can catalyse chemical reactions without changing themselves. The enzyme lipase breaks down the fat in dairy products such as full-cream milk for people who are lactose intolerant. Lipase acts on its specific substrate‚ lipids produces fatty acids. If enzyme concentration increases‚ random collisions between the substrates and active sites of enzyme increase due to the increasing
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Case study A blue paint layer sample was collected from the wall decorations of the mortuary temple of Ramesses III (Medinet Habu. This temple is one of the largest memorial temples in Egypt. It measures 320 m in length (east to west) and about 200 m in width (north to south)‚ built with order of the Pharaoh Ramesses III (c.1184–1151 BC the 20th Dynasty). The micro-XRF spectrometer (SPECTRO‚ COPRA model) used to analyze the sample includes a side-window X-ray tube with Mo anode (Oxford Instruments
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