of substrate can fit to the enzyme’s active site. The action of enzymes is reversible and enzymes can be reused. This means that an enzyme can catalyze lots of substrates. Materials Starch solution‚ sodium chloride solution‚ amylase solution‚ iodine solution Apparatus Test tubes‚ droppers‚ white tile‚ stopwatch‚ water bath controller Procedure 1. A water bath is set up at 30°C. 2. 3cm3 starch solution added into a test tube labeled A. 1cm3 of sodium solution is added into the test tube
Free Enzyme
curcas seed fats and oil‚ and (2) Identification and characterization of unknown oil. After the fats and oil was extracted from seeds of Jathropa curcas‚ the percent yield was reported as 40-41%. The specific gravity‚ refractive index‚ acid value‚ iodine value‚ peroxide value‚ and the saponification value of the unknown oil sample were determined. The computed values were 1.05‚ 1.5765 ± 5.7735 x 10-5‚ 0.328 ± 2.31 x 10-3 mg acid / g oil‚ 10. g I2 / 100 g oil‚ 22 ± 2 ml / g oil‚ and 208 ± 8.49 mg
Premium Fatty acid
then in the presence of Iodine‚ their colors if the test sample contains sugars‚ then the iodine will change from yellow-brown to blue-black. 4. If the test samples show the presence of lipids‚ the when the substance is placed onto a brown paper bag and sits for 10 minutes‚ then a translucent or opaque spot will appear. Materials: * 8 test tubes * test tube rack * test tube holder * glass stirring rod * sharpie marker * how water bath * iodine solution * brown
Premium Carbohydrate Protein Glucose
gram-positive. The gram staining procedure consists of fixing a colony of bacteria onto a slide and then flooding the colony with various chemicals. First‚ crystal violet dye was dropped onto the bacterial cells‚ staining gram-positive cells purple. Iodine was added to fix the violet dye into place and then ethanol was used to wash the dye off the unstained cells. Finally‚ a red dye called safranin was used to stain any gram-negative cells present so that they were visible. Once the gram stain procedure
Premium Gram staining Bacteria Staining
Manual. Vol. 2013-2014. Plymouth: Hayden-McNeil. 32-35. Data/Results: Part A: In the potassium iodide solution‚ I think there were potassium atoms as well as iodine atoms. In the lead nitrate solution‚ I think there were lead and nitrate ions. The potassium atoms and the lead atoms can be classified as cations‚ since they are metals. The iodine atoms and the nitrate ion can be classified as anions since they are nonmetals. Upon mixing‚ the solution turned into a cloudy yellow color. Evidence that a
Premium Management Organization Strategic management
determined by analyzing the amount of iodine (I2) formed. Two chemical reactions are useful to determining the amount of iodine is produced. I2(aq) + 2S2O32-(aq) 2I-(aq)+S4O62-(aq) (3) I2(aq) + starch I2 *starch(blue complex) (4) Reaction (4) is an iodine-starch reaction‚ used solely to determine when the production of iodine is occurring by turning a clear colorless solution to a blue color. Without reaction (4) it would be very difficult to determine how much iodine is being produced‚ due to how
Premium Standard deviation Scientific method Statistics
The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed
Free Enzyme Starch
chemical reaction but does not become part of the end product. Amylase digests starch by catalyzing hydrolysis‚ which is splitting by the addition of a water molecule. The presence and absence of starch can be confirmed by several tests such as the iodine test‚ Benedict’s and Fehling’s test. In general‚ a blue-black color indicates the presence of starch. The objectives of this experiment are to examine the enzymatic activity and specificity of salivary amylase depending on changes temperature and
Premium Enzyme Buffer solution Starch
chloroplasts‚ where the photosynthesis occurs. During experiment‚ two leaves were tested for the presence and localization of starch. One leaf was a light-grown plant with mask‚ and the other was a dark-grown plant with mask. Both leaves were soaked in iodine solution and then rinsed in order to observe the pattern of staining. According to the result‚ the light-grown leaf’s masked areas had no blue-black coloration and the unmasked area turned blue-black in color. The blue-black coloration of the leaf
Premium Photosynthesis Leaf Carbohydrate
not taste any substance in this experiment * Iodine solution and some powders are hazardous. * Wash hands when you have finished. Materials * Eye protection * Well plate * 6 small toothpicks for stirring each powder * 6 white powders: icing sugar‚ cornstarch‚ Aspirin powder‚ baking soda‚ baking powder‚ and the mystery powder * Scoopula * Test solutions in dropper bottles: water‚ universal indicator‚ vinegar‚ and iodine solution
Premium Starch Water Sodium bicarbonate