By the end of this lab‚ the student should be able to: Identify the functional groups for each of the biomolecules that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Brown Bag test‚ Sudan III/IV test‚ and the Biuret’s Test. Describe the mechanism of reaction for: Benedict’s test‚ Iodine test‚ Sudan III/IV test‚ and the Biuret’s Test. Interpret the results when presented with data for each of the biochemical tests. Design experiments to identify biomolecules using
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In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed. Thus the diameter of the clear zone can show the activity of the amylase. In this experiment
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by titration between vitamin C (ascorbic acid) & iodine solution. Hence‚ compare this data with the manufacturer ’s specification. Principle In this analysis‚ certain amount of iodine solution‚ which is known in excess‚ is added to acidified ascorbic acid solution. Brown iodine can be easily reduced by acidified ascorbic acid to form colourless iodide ion: ← Equation I The excess iodine is then back titrated by standard sodium thiosulphate
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iodometry- the winkler’s method. Apparatus: volumetric pipette‚ 3 conical flask‚ burette‚ burette clamp‚ Pasteur pipette‚ reagent bottle‚ conical flask stopper‚ retord stand‚ white tile Materials: 2 ml manganese sulphate solution‚ 2 ml alkaline-iodine solution‚ 0.025M sodium thiosulphate solution‚ 2ml concentration sulphuric acid‚ starch solution Procedure 1. When sampling water‚ care must be taken to ensure that a good representative sample of the water to be analyzed is obtained. For most
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the unknown solution. The macromolecules being tested in this lab were carbohydrates (monosaccharides and polysaccharides) and proteins. To identify the presence of these macromolecules in a substance‚ three different tests were performed. Lugol ’s iodine solution was used for identification of starch and glycogen - polysaccharides - in the twelve solutions. A positive outcome of the test results in a colour change; blue-black in the presence of starch and a red-brown in the presence of glycogen. A
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G-250 * Bradford Reagent * Bovine Serum Albumin = BSA * Reagent blank = test tube #1 = 0mL BSA stock solution‚ 1mL distilled H2O * 595nm * Plot A595 vs concentration of BSA Experiment 4: Enzymatic Activity of Salivary Amylase * Iodine test – presence of starch * 1/t vs temperature/pH Experiment 5: General and Specific Tests for Carbohydrates | Reagents | Principle | Test for | Positive Result | Negative Result | Molisch | Molisch Reagent ‚Conc. H2SO4 | hydrolysis‚ dehydration
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Giancarlo Vidal CHM 1033 10/16/14 “Role of the Trace Elements - Iron‚ Zinc‚ Chromium‚ Copper‚ Iodine‚ and Selenium in the human body” Trace elements are necessary by the body for normal growth‚ development and physiology. Even though many of the elements are required in only very small quantities‚ they do play a very significant role in keeping the body functioning efficiently. A surplus or shortage of the trace elements in our bodies can equally threaten human health. Iron is an integral part of
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fructose‚ maltose‚ sucrose‚ lactose‚ |Molisch reagent | |agar-agar‚ gum arabic‚glycogen‚ cotton‚ |I2 in KI solution (Lugol’s iodine reagent) | |starch |Benedict’s reagent | |10% HOAc solution‚
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these factors are absent‚ photosynthesis cannot occur. Materials required Materials list Geranium‚ begonia‚ or impatiens plants (entirely green leaves)‚ coleus with variegated leaves (green and white colored)‚ hot plates and hot-water bath‚ Lugol’s iodine solution in dropper bottles‚ 250-mL beakers‚ 100-mL beakers‚ tongs‚ foreceps‚ Petri dishes‚ glass-marking pencil‚ 70% alcohol Method PART I - Effects Of Light And Dark
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October 2014 Periodicity of the Halogens Goal: To design and carry out an experiment that will give enough data to rank the three halogens from highest to lowest electron affinity. Background: The three aqueous solutions given in this experiment are Iodine‚ Bromine‚ and Chlorine; they are all non-polar and dissolve easily in Hexane which is also non-polar. When there is a color change in a mixture that means that it reacted and that there was a double displacement reaction when there is no change then
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