By the end of this lab‚ the student should be able to: Identify the functional groups for each of the biomolecules that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Brown Bag test‚ Sudan III/IV test‚ and the Biuret’s Test. Describe the mechanism of reaction for: Benedict’s test‚ Iodine test‚ Sudan III/IV test‚ and the Biuret’s Test. Interpret the results when presented with data for each of the biochemical tests. Design experiments to identify biomolecules using
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together). As the reaction progresses‚ less starch will be present and more sugar (maltose) will be present. In lab‚ the activity of amylase was observed by using iodine. Remember‚ iodine reacts with starch to form a dark brown/purple color. As amylase breaks down starch‚ less and less starch will be present and the color of the solution (if iodine is added) will become lighter and lighter Rates of enzyme-catalysed reactions depend on pH and temperature. The optimum temperature is about 40oC because the
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tests performed were iodine testing for starch‚ Benedict’s test for glucose‚ the grease spot test for lipids‚ and the CuSO4’s test for protein. The pH indicator’s detection is based upon observing a chemical change that takes place most often a change in color. The Starch test was used for the presence of starch. In this instance‚ the distilled water is a negative control for the test‚ and a known glucose solution is a positive control. This was a simple test in which iodine is added to a given
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sugar in it by adding Benedict’s solution and heating it‚ there would be a color change if a reducing sugar is present‚ or it will remain blue (no reducing sugar). The objective of the Starch test was to test for starch in substances by using Iodine. The iodine will cause a substance to turn to a dark blue color if it is positive for starch. The objective for the Dye test was to test for lipids. When mixed with water and the tested substance‚ a positive result will occur in it being separated from
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In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed. Thus the diameter of the clear zone can show the activity of the amylase. In this experiment
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temperature. * Temperature influences time taken for iodine solution remains yellowish. No full marks will be given if students did not meet the 2 criteria of answer and the word ‘different’ / ‘influence’ is not specific. Students must try to avoid these words. Accurate response (3 marks): P1: Temperature of water bath P2: Time taken for iodine solution to remain yellowish (Horizontal observation) * At temperature 5˚C‚ time taken for iodine solution remains yellowish is 12 minutes. * At
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by titration between vitamin C (ascorbic acid) & iodine solution. Hence‚ compare this data with the manufacturer ’s specification. Principle In this analysis‚ certain amount of iodine solution‚ which is known in excess‚ is added to acidified ascorbic acid solution. Brown iodine can be easily reduced by acidified ascorbic acid to form colourless iodide ion: ← Equation I The excess iodine is then back titrated by standard sodium thiosulphate
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likely to suffer from severe acne. That is because the increase in growth hormone which causes the sebaceous glands in the skin become clogged. 3. Iodine Iodine may be one factor in the appearance of acne‚ such as skin oil glands which can irritate skin that is prone to acne. Iodine can be found in fish and shellfish because sea water contains iodine. In addition other sources comes from iodized salt which is a component of several types of canned food. 4.Caffeine and Alcohol Caffeine is considered
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Differentiate between a differential stain and a special stain (how are they different‚ don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure if the alcohol step in the gram
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If Benedict solution is added to corn oil‚ then it will turn. If Benedict solution is added to glucose‚ then it will turn. If Benedict solution is added to gelatin‚ then it will turn. If Benedict solution is added to butter‚ then it will turn. If iodine is added to starch‚ then it will turn. If Benedict color is added to apple juice‚ then it will turn. If any organic substance is tested with distilled water‚ then nothing will happen. Materials: -12 test tubes -Test
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