Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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Civil Engineering Subject : Structural Analysis I - Laboratory Report Laboratory Venue : HKIVE (Tsing Yi)‚ Room CL02 Date & Time : 15 October 2001‚ 19:00 to 20:15 Experiment No. 1 : Column Buckling Test Objective: 1. To study the effect of support conditions on the load‚ carrying capacity of a slender column. 2. To compare the experimental buckling loads Pcr of test specimens with those predicted by the Euler equation. Apparatus: 1. SM 105 strut apparatus (Issuing Voucher: 0203141 & Inventory
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When people hear candy chromatography‚ they don’t know what to think about it. They of course know that it had something to do with candy. But just don’t know what. Candy chromatography is fun and simple. Candy Chromatography is a fun project‚ plus it has to do with candy. Candy Chromatography is an experiment to find out the true colors of the dyes in candy. When you put a skittle or an M&M in your hand in hot weather‚ the candy tends to bleed the color. And sometimes it shows more than one color
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Gas Chromatography Analysis of Product Mixtures Gas Chromatography Guidelines‚ Handout. Introduction Gas chromatography is a technique used to analyze chemical compounds that can be vaporized and separated in a gas phase column. Once separated‚ the analyzed substance is passed through a detector and data is obtained. The samples that we are going to analyze are: the EtOAc from Simple distillation‚ the Fraction 1‚ Fraction 2‚ and Fraction 3 from the Fractional Distillation. Experimental Procedure
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What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
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Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
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Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3) M.P
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The purpose of this lab was to use gas chromatography to determine the relative amounts of the three components of gas products from the dehydration reaction of 1- and 2-Butanol with sulfuric acid and the dehydrobromination reaction of 1- and 2-Bromobutane with potassium tert-butoxide. E1 reactions are two-step unimolecular reactions. The first step of an E1 reaction occurs when a leaving group‚ which consists of a weak base‚ leaves an organic compound‚ leaving an intermediate carbocation. In the
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Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography
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