Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase‚ which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds‚ causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound’s partition coefficient result in differential retention
Premium Chromatography Analytical chemistry Thin layer chromatography
Fractional Distillation & Gas Chromatography Exp. 1 Pre-Lab: 1) When two substances whose molecules are very similar from a liquid solution‚ the vapor pressure of the mixture related to vapor pressure of the pure substance. Also it could be defined as a two liquid are ideal solution when they don’t react with each other and they make no association. 2) Are a mixture of at least two different liquid‚ and known also as a mixture of two or more liquid in such away that its component
Premium Distillation Chemical compound
General Conclusion: These experiments helped us understand the importance of molecular polarity. This knowledge can now be used for the future to predict‚ for example‚ if two substances will mix or not. We can now do this by knowing that polar substances only mix with other polar substances‚ while non-polar substances only mix with other non-polar substances. This concept can be used to predict if a substance will be soluble in a specific solvent. The concepts of solubility and conductivity of a
Premium Chemical polarity Solubility Water
Conclusion Need to do more of promotional strategies to attract more of female consumers with like of limited edition specially for females in their existing models or for new models in this edition just by giving few simples thing which females like in the car for example- pink colour‚ soft-toys‚ trendy seat covers‚ etc. Furthermore‚ The Asia Pacific region is becoming increasingly crucial to global automotive sales. Although it has been overshadowed by China ’s rapid growth in recent years‚
Premium Marketing
CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
Premium Thin layer chromatography Chromatography Solvent
It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
Premium Oxygen Thin layer chromatography Ethanol
Standing over fifty-eight feet high is the Marble Column from the Temple of Artemis at Sardis‚ known as one of the seven wonders of the world. The marble column that stands tall at the Metropolitan Museum of Art is different from the rest found at the site of the temple because the capital is slightly smaller than the rest. This indicates that it did not belong to the outer colonnade. The columns at the Temple of Artemis are found to be ionic orders‚ being one of the three different types of orders
Premium Ancient Rome Corinthian order Ionic order
Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
Premium Chromatography Protein Analytical chemistry
Gas chromatography (GC) is a type of chromatography that uses a carrier gas as the mobile phase and a column as the stationary phase. The sample is injected into the instrument and is heated until the sample‚ which includes both analyte and solvent‚ boils. The analyte must have a relatively low boiling point in order to be to be pushed through the column along with the carrier gas‚ helium.1 The column used in the experiment was a non-polar Agilent HP-5 column (specific phase found
Premium Orange Citrus Essential oil
distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
Premium Distillation Gas Water