Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Thin-Layer Chromatography of Analgesics Abstract: In this experiment thin-layer chromatography (TLC) was used to determine the composition of an unknown mixture of analgesics given five references of AC‚ ASP‚ CAF‚ IBU‚ SAL‚ an unknown consisting of a mixture of three of the references‚ and two unknowns (6‚23) containing a single reference compound. The composition of the unknown mixture and two single unknowns were determined by spotting the reference compounds and the unknown compounds on two
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Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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Chromatography Experiment of the Drosophila Eye Pigmentation Pathway Introduction: How can the use of chromatography help explain single gene mutations of the drosophila melanogaster bio-synthetic pigment pathway? This experiment was meant to help connect the mode of inheritance with different eye color mutations and pinpoint where they occur in the pigment pathway. The drosophila eye color is a result of two bio-synthetic eye color pathways‚ this is expressed as a trait or phenotype‚ a multigene
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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In conclusion to the distillation lab‚ I think our data shows that our experiment went well. Our graph resembles the graph in the lab handout (graphs attached to lab notebook sheets). The fractional distillation shows the sharp slope between when the cyclohexane burns off and mostly only toluene remains. The microscale graph seems to be accurate because it shows the large jump where the cyclohexane is burned off. To view the apparatuses used in the experiment look at the attached pictures. To
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Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the column is referred
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CVEN9802 Structural Stability Beam Columns Chongmin Song University of New South Wales Page 1 CVEN 9802 Stability Outline • Effective Length Concept • Beam-Column with Distributed Load • Column with Imperfection • Southwell Plot • Column Design Formula Page 2 CVEN 9802 Stability Fundamental cases of buckling PE EI 2 L 2 2 2.045 EI P 4 EI Pcr cr 2 L2 L 2 Pcr 2 EI 4L 2 PE 2 EI L2 Page 3 CVEN 9802 Stability What is
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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