Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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understand that because substances have different properties‚ such as mass‚ that can be separated by chromatography. In our experiment‚ we chose 4 different color source to separate in water. We made sure that all the sources were water soluble because only polar substances will dissolve in water. We chose a black wet-erase marker‚ a red marker‚ a green marker‚ and mixed food coloring to test. The chromatography paper was split into 4 sections‚ about 2 cm above the bottom edge and evenly spaced out. Each
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the Kool-Aid was correct. However‚ solely based upon the Rf values‚ the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However‚ the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because one color band was of a blue tint and the other‚ a yellow tint. Therefore‚ based on this qualitative data‚ the dyes in the green Kool-Aid are
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in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front (mm) Rf Value Color A 6.0 57.0 0.22 Bluish green B 17.5 56.0 0.105 Dark green blue C 4.0 57.0 0.070 Greenish blue D 4.0 47.0 0.085 Green E 10.5 56.5 0.186 Light blue Figure 1: Chromatography- TLC sheet From left to right- acetic acid‚ citric acid‚
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Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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1 TOPIC 4 Course Learning Outcomes Able to : 1. Explain the fundamental concepts & theories of separation techniques in SFC & SFE. 2. Sketch‚ label the schematic diagrams & discuss the function of each component in SFC & SFE. 3. Identify the strength & limitations of SFC & SFE technique. 4. Suggest and justify the most suitable & efficient separation technique to be employed for an analysis. 2 What is supercritical fluid? 3 Critical temperature (Tc) for any substance is a temperature
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term ( major‚ minor‚ and middle terms) can be represented by a circle. | | C. Since a syllogism is valid if and only if the premisses entail the conclusion‚ diagramming the premisses will reveal the logical geography of the conclusion in a valid syllogism. If the syllogism is invalid‚ then diagramming the premisses is insufficient to show the conclusion must follow. | | D. Since we have three classes‚ we expect to have three overlapping circles. | 1. The area in the denoted circle represents
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JUMPING TO CONCLUSIONS IS WRONG People in today’s society tend to jump to the wrong conclusions about others. Jumping to the wrong conclusions can be very hurt full to other people. In my opinion I think that people should stop jumping to conclusions and take the time to get to know someone. . These are the three main ones that I see the most are peoples appearance‚ race and religion‚ location. People are often jumping to the wrong conclusions about someone’s appearance. They would see a person
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Recommendations & Conclusions We had looked at all the characteristic of the business environment together with the market segment of Indonesia. We studied the marketing mix such as product‚ pricing‚ placement and promotion. These are the recommendations and conclusions that we have conclude in our proposal. Java is the most populous and the most developed island in the Indonesian archipelago. The island is politically divided into the provinces of West Java‚ East Java and Central Java. The
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