Alkaline Phosphatase was isolated using various techniques. BCIP spot test was performed after the anion exchange chromatography was done and the fractions were collected. The picture of BCIP spot test of the 20 fractions can be seen in figure 1. The intensity of each spot was different from each other. The more intense color the spot was‚ the more concentrate the AP was. The fractions were chosen to combine together based on the intensity‚ which made the stage 4 enzyme. Different volumes in each
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Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it
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Lab #3 Separating Metal Cations by Paper Chromatography Introduction/Purpose: In chemistry‚ it is important to effectively analyze the components in a given mixture. In order to do so‚ chemists will analyze a mixture either qualitatively or quantitatively using different methods and equipment. Qualitative analyses are subjective‚ and only gather “non-numerical” data such as the color‚ appearance‚ and smell of the components. This data
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soft soils using geogrid encased stone columns Joel Gniel 1‚ Abdelmalek Bouazza* Monash University‚ Department of Civil Engineering‚ Building 60‚ Melbourne‚ Victoria 3800‚ Australia a r t i c l e i n f o a b s t r a c t Article history: Received 16 May 2008 Received in revised form 4 November 2008 Accepted 5 November 2008 Available online 19 January 2009 In recent years‚ geotextile encasement has been used to extend the use of stone columns to extremely soft soils. Although the
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In this lab we tested to see if the amount of sunlight exposed to the basil seed‚ would affect the growth. The null hypothesis for this experiment was that there would be no statistical difference between the green‚ yellow‚ and red basil seeds’ growth. Three alternate hypotheses are: If we expose the green seed to 48 hours of sunlight and the yellow seed to 24 hours of sunlight‚ then the green seed with grow more than the yellow. The opposite of this hypothesis would alternate 2. Another alternate
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Chemistry 266 Dominick Armstrong 10/10/2012 Acid-Base Titration Introduction Acid and bases are two important classes of compounds that react to form a salt and water. When mixing acids and bases a precise amount of the base must be added in order to reach the equivalence point. At this point‚ one reactant has been exactly consumed by the addition of the other reactant. When performing chemical reactions chemist use a process called titration to determine the equivalence point of the
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Is Higher Education Worth the Price? The question of whether or not higher education is actually “worth it” is an entirely subjective on. To objectively determine if higher education will give a high return on investment‚ one needs to first understand what they are hoping to gain out of the entire experience. Higher education does worth the price because it helps students to build their self-fulfillment‚ provide more monetary opportunities and reach their final goal . In the United States‚ for
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Why Conclusion is Important in Term Papers Writing Conclusion is the last part of any type of paper‚ usually a college term paper comprised of three main parts introduction‚ body and finally conclusion. The conclusion of a term paper should be well written because as it is the last part of a paper so it should have a lasting impression on readers and audience. It is an integral part of any study as it sums up important arguments and its evidence to support and proves the thesis statement. Importance
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Look again at Act V Scene 4. To what extent do you find it a satisfactory conclusion to the play? Act V Scene 4 is a satisfactory conclusion to the play Much Ado About Nothing because it includes certain conventions of comedy; an example being the happy ending when Hero and Claudio are reunited and Benedick and Beatrice’s love is made public. There is dramatic irony within this scene as deception is used as Hero pretends to be her ‘cousin’‚ which creates tension as the audience wonder whether
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Marcel Pagnol- Le Chateaux de ma mere Discuss the reasons for the sudden change in the conclusion of the book “ Le chateaux de ma mere” by Marcel Pagnol is a memorable‚ enjoyable text in which the story takes an abrupt turn at the conclusion of the book. Pagnol utilises the majority of the text to develop the story of months and a series of events‚ however he majestically creates a sudden change in the final components of the book which span a period of decades. It is not solely the series
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