Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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Gas chromatography (GC) is a type of chromatography that uses a carrier gas as the mobile phase and a column as the stationary phase. The sample is injected into the instrument and is heated until the sample‚ which includes both analyte and solvent‚ boils. The analyte must have a relatively low boiling point in order to be to be pushed through the column along with the carrier gas‚ helium.1 The column used in the experiment was a non-polar Agilent HP-5 column (specific phase found
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he backbone (vertebral column) is the central axis of the skeleton. It extends from the base of the skull to the bottom of the pelvis. It consists of 26 individual bones grouped into five separate regions; The vertebrae‚ twelve thoracic vertebrae‚ five lumbar vertebrae‚ one sacral bone and one coccygeal bone. (Cinnamon L. VanPutte‚ Jennifer L. Regan‚ Andrew F. Russo‚ McGraw-Hill‚ 2010.) The Vertebral column also host major curvatures; The sacral and thoracic both concaving anteriorly and the lumbar
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distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
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foreground the composition‚ wearing a flowing white skirt and a corset of white straps that wrap around her shoulders and upper body. Kahlo’s breasts are exposed and the front of her upper body is bisected‚ revealing that “her spinal column is replaced by a broken Doric column.” In addition‚ several nails protrude from the skin on her face‚ arms‚ torso‚ and legs. In the background‚ a blue sky with white clouds hangs over the cracked‚ fissured landscape where Frida stands. Unlike The Two Fridas‚ this
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This lab is an opportunity to observe‚ study‚ and learn about ecosystems and how the environment sustains life but in a much simpler and smaller scale which is called Eco Columns. The environmental conditions in the Eco Column could sustain life for several weeks‚ therefore over the course of the several weeks we will observe and study the interconnections between each ecosystems. A self sustaining requires components such as energy flow and chemical cycle. This experiment will evaluate our knowledge
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Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
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Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing the most amount of fluorescence
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ANALYSIS OF MOUTHWASH Background Information: Commercial mouthwashes are mixtures of water‚ alcohol‚ dyes‚ flavorings‚ and other compounds. In this experiment‚ you will use gas chromatography to determine the alcohol content of the mouthwash. Since the volumes of alcohol and water are not additive when mixed‚ a calibration curve must be used. It will provide the correction factor needed for alcohol-water mixtures. The calibration curve will be prepared by placing a known amount of alcohol
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How To Obey One’s Father‚ While Being True To Love and Oneself By: Ophelia Posted: 11/2/2013 12:58pm EST When do we know when to listen to ones heart‚ but at the same time make the right choices by listening to others voices? Do we listen to the demands of our guardians? or is love what drives us to make better choices? Some of us are blinded by love‚ and I for one have experienced the treachery of being bashed by my own lover. I did not see what should have been sawn‚ because I was in the meadows
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